TÜBİTAK, The Scientific & Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering & Biotechnology Institute, 41470 Gebze, Kocaeli, Turkey.
Republic of Turkey Ministry of Food Agriculture & Livestock, General Directorate of Food & Control, Turkey.
Biotechniques. 2019 Jun;66(6):261-268. doi: 10.2144/btn-2018-0143. Epub 2019 May 24.
Antibodies are the basic components of immunoanalytical systems used for detection of a wide range of analytes. Although there are some ground rules for antibody selection, analyte- and assay-specific criteria are the ones that determine the ultimate success of the immunoassays. In this study, we introduced an effective antibody selection procedure for the development of immunoaffinity columns for aflatoxins. The designed scheme puts emphasis on solvent- and matrix-related characterization steps and was used to comparatively evaluate eight monoclonal antibodies. The selected antibody was tolerant to 40% methanol, 20% acetonitrile, 30% acetone and 40% ethanol and did not interact with corn, red pepper or hazelnut extracts. Immunoaffinity columns developed with the selected antibody were validated by 15 independent aflatoxin analysis laboratories.
抗体是用于检测广泛分析物的免疫分析系统的基本组成部分。尽管抗体选择有一些基本规则，但分析物和测定特异性标准是决定免疫分析最终成功的关键。在这项研究中，我们为黄曲霉毒素免疫亲和柱的开发引入了一种有效的抗体选择程序。该设计方案强调了与溶剂和基质相关的表征步骤，并用于比较评估了八种单克隆抗体。所选抗体耐受 40%甲醇、20%乙腈、30%丙酮和 40%乙醇，并且与玉米、红辣椒或榛子提取物不相互作用。用所选抗体开发的免疫亲和柱由 15 个独立的黄曲霉毒素分析实验室进行了验证。
