R&D Center, NH Foods Ltd., 3-3 Midorigahara, Tsukuba, Ibaraki 300-2646, Japan.
Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, 2393 Ikenobe, Miki, Kagawa 761-0795, Japan.
J Food Prot. 2019 Sep;82(9):1472-1478. doi: 10.4315/0362-028X.JFP-19-036.
We combined immunoaffinity column (IAC) and enzyme-linked immunosorbent assay (ELISA) methods to develop a rapid method to analyze total aflatoxin (AF) in foods, using a large number of samples. Using newly developed monoclonal antibodies, with high cross-reactivity and high organic solvent tolerance, we developed the IAC-ELISA method. Our IAC-ELISA method showed a good correlation with the high-performance liquid chromatography method for corn samples spiked with total AF. Certain food samples, such as chili powder, chocolate, green coffee beans, and roasted coffee beans, are difficult to measure owing to their matrices, which affect ELISA adversely. Our IAC-ELISA method clearly improved the recovery rates (79 to 109%) compared with the ELISA method (97 to 164%) for four food samples. Our method is simple and quick; thus, it may be ideal for routine inspections.
我们结合免疫亲和柱（IAC）和酶联免疫吸附测定（ELISA）方法，开发了一种快速分析食品中总黄曲霉毒素（AF）的方法，该方法可用于大量样本。我们使用新开发的具有高交叉反应性和高有机溶剂耐受性的单克隆抗体开发了 IAC-ELISA 方法。我们的 IAC-ELISA 方法与高效液相色谱法对玉米样品中总 AF 的加标回收率具有良好的相关性。某些食品样品，如辣椒粉、巧克力、绿咖啡豆和烤咖啡豆，由于其基质会对 ELISA 产生不利影响，因此难以测量。与 ELISA 方法（97%至 164%）相比，我们的 IAC-ELISA 方法明显提高了四种食品样品的回收率（79%至 109%）。我们的方法简单快捷；因此，它可能是常规检测的理想选择。
