Christian Doppler Laboratory for Monitoring of Microbial Contaminants, Department for Farm Animal and Public Health in Veterinary Medicine, University of Veterinary Medicine, Vienna, Austria.
Unit of Food Microbiology, Institute of Food Safety, Food Technology and Veterinary Public Health, Department for Farm Animal and Public Health in Veterinary Medicine, University of Veterinary Medicine, Vienna, Austria.
Appl Environ Microbiol. 2019 Jul 1;85(14). doi: 10.1128/AEM.00766-19. Print 2019 Jul 15.
Detection of pathogens is crucial in food production areas. While it is well established, swabbing as a state-of-the-art sampling method offers several drawbacks with respect to yield, standardization, overall handling, and long-term monitoring. This led us to develop and evaluate a method that is easier to use at a lower cost and that should be at least as sensitive. After evaluating sundry promising materials, we tested text-marking paper stickers for their suitability to take up and release with their nonsticky paper side over a 14-day time period using quantitative PCR. The recovery rate was similar to that in previous studies using conventional swabs, and we also confirmed the feasibility of pooling besides resilience to cleansing and disinfection. In a proof-of-concept experiment that sampled several locations, such as door handles, the occurrences of and were determined. The results suggest that the presented sticker system might offer a promising cost-effective alternative sampling system with improved handling characteristics. As a ubiquitous bacterium, has a propensity to enter food production areas inadvertently via fomites such as door handles and switches. While the bacterium might not be in direct contact with the food products, knowing the microbial status of the surroundings is essential for risk assessment. Our investigation into a novel quantitative PCR (qPCR)-based sampling system with the highest sensitivity and ability to monitor over long periods of time, yet based on paper, proved to be cost-effective and reasonably convenient to handle.
在食品生产区域，病原体的检测至关重要。虽然拭子采样作为一种先进的采样方法已经得到广泛应用，但它在产量、标准化、整体处理和长期监测方面存在一些缺点。这促使我们开发并评估了一种更易于使用、成本更低的方法，并且该方法至少应具有相同的灵敏度。在评估了各种有前途的材料之后，我们测试了文本标记纸贴纸在使用定量 PCR 检测 14 天内从其非粘性纸侧吸收和释放 的能力。回收率与之前使用传统拭子的研究相似，我们还证实了除了耐清洁和消毒之外，还可以进行混合。在一个采样了多个位置（如门把手）的概念验证实验中，确定了 和 的存在情况。结果表明，所提出的贴纸系统可能提供了一种有前途的具有成本效益的替代采样系统，具有改进的处理特性。作为一种普遍存在的细菌， 会通过门把手和开关等媒介物无意中进入食品生产区域。虽然该细菌可能不会直接与食品接触，但了解周围环境的微生物状况对于风险评估至关重要。我们对一种基于新型定量 PCR (qPCR) 的采样系统进行了研究，该系统具有最高的灵敏度和长时间监测的能力，并且基于纸张，结果证明该系统具有成本效益且处理方便。
