在亚麻（Linum usitatissimum）木质素生物合成过程中，对 R2R3、3R-和 4R-MYB 转录因子进行全基因组鉴定和表达分析。

Genome-wide identification and expression analysis of R2R3, 3R- and 4R-MYB transcription factors during lignin biosynthesis in flax (Linum usitatissimum).

机构信息

Department of Genetics Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, 31441 Dammam, Saudi Arabia.

出版信息

Genomics. 2020 Jan;112(1):782-795. doi: 10.1016/j.ygeno.2019.05.017. Epub 2019 May 23.

DOI:10.1016/j.ygeno.2019.05.017

PMID:31128265

Abstract

MYB transcription factors (TFs) have vital roles in regulating lignin or fiber development. Flax (Linum usitatissimum) is known as one of the plants with high fiber production capacity. However, no studies have been conducted to identify and characterize MYB TFs in the flax genome. Results showed that flax genome harbours 167 R2R3, seven 3R, and one 4R-type MYB TFs. 22 MYB genes (%13) were estimated to be tandem duplicated dated around 13.3-86.98 Mya. 130 flax MYB members have apparent orthologous with Arabidopsis, in which 17 R2R3 MYBs are associated with lignin biosynthesis. MYB062, MYB072, MYB096, MYB141, and MYB146 genes were up-regulated in tissues having higher lignin production capacity. In opposite, MYB012 and MYB113 genes were down-regulated which points out the involvement of those genes in the lignin biosynthesis mechanism. This comprehensive study can provide a basis for understanding the role of MYBs in fiber or lignin production in flax.

摘要

MYB 转录因子（TFs）在调节木质素或纤维发育方面起着至关重要的作用。亚麻（Linum usitatissimum）是已知具有高纤维生产能力的植物之一。然而，目前尚未有研究对亚麻基因组中的 MYB TFs 进行鉴定和特征描述。研究结果表明，亚麻基因组包含 167 个 R2R3、7 个 3R 和 1 个 4R 型 MYB TFs。22 个 MYB 基因（占 13%）被估计为约 13.3-86.98 Mya 时发生的串联重复。130 个亚麻 MYB 成员与拟南芥有明显的直系同源性，其中 17 个 R2R3 MYBs 与木质素生物合成有关。MYB062、MYB072、MYB096、MYB141 和 MYB146 基因在木质素产量较高的组织中上调表达。相反，MYB012 和 MYB113 基因下调表达，这表明这些基因参与了木质素生物合成机制。这项全面的研究可为理解 MYBs 在亚麻纤维或木质素生产中的作用提供基础。