Fu Yi, Lakowicz Joseph R
Center for fluorescence spectroscopy, University of Maryland School of Medicine, 725 W. Lombard Street, Baltimore, MD 21201, USA.
Laser Photon Rev. 2009 Feb;3(1-2):221-232. doi: 10.1002/lpor.200810035. Epub 2009 Feb 19.
In recent years there has been a growing interest in the interactions of fluorophores with metallic nanostructures or nanoparticles. The spectra properties of fluorophores can be dramatically modified by near-field interactions with the electron clouds present in metals. Near-field interactions are those occurring within a wavelength distance of an excited fluorophore. These interactions modify the emission in ways not seen in ensemble fluorescence experiments. In this review we provide an insightful description of the photophysics of metal plasmons and near-field interactions. Additionally, we summarize recent works on single-molecule studies on metal-fluorophore interactions and suggest how these effects will result in new classes of experimental procedures, novel probes, bioassays and devices. The spectral properties of single fluorophores can be dramatically altered by near-field interactions with the electron clouds presented in metals.
近年来，人们对荧光团与金属纳米结构或纳米颗粒之间的相互作用越来越感兴趣。荧光团的光谱特性可通过与金属中存在的电子云的近场相互作用而发生显著改变。近场相互作用是指在激发荧光团的一个波长距离内发生的相互作用。这些相互作用以整体荧光实验中未观察到的方式改变发射。在本综述中，我们对金属等离子体激元和近场相互作用的光物理进行了有见地的描述。此外，我们总结了近期关于金属 - 荧光团相互作用的单分子研究工作，并提出这些效应将如何导致新型实验程序、新型探针、生物测定法和器件的产生。单个荧光团的光谱特性可通过与金属中呈现的电子云的近场相互作用而发生显著改变。
