Zhang Jian, Fu Yi, Lakowicz Joseph R
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201.
J Phys Chem C Nanomater Interfaces. 2007 Jan 11;111(1):50-56. doi: 10.1021/jp062665e.
We examined the effect of a metallic silver particle on Förster resonance energy transfer (FRET) between a nearby donor-acceptor pair. A donor- labeled oligonucleotide was chemically bound to a single silver particle and then an acceptor- labeled complementary oligonucleotide was conjugated by hybridization. The photophysical behavior of FRET between the donor-acceptor pair on the metal particle was investigated using both ensemble emission spectra and single- molecule fluorescence detections. Both the emission intensities and lifetimes indicated an enhanced FRET efficiency due to the metal particle. This interaction led to an increase in the Förster distance for energy transfer from 8.3 to 13 nm. The rate constant of FRET near the silver particle was 21-fold faster than that of unbound donor-acceptor pair. These results suggest the use of metal-enhanced FRET for measuring proximity of large biomolecules or for energy transfer based assays.
我们研究了金属银颗粒对附近供体-受体对之间荧光共振能量转移（FRET）的影响。将供体标记的寡核苷酸化学结合到单个银颗粒上，然后通过杂交结合受体标记的互补寡核苷酸。使用总体发射光谱和单分子荧光检测研究了金属颗粒上供体-受体对之间FRET的光物理行为。发射强度和寿命均表明由于金属颗粒导致FRET效率提高。这种相互作用导致能量转移的Förster距离从8.3 nm增加到13 nm。银颗粒附近FRET的速率常数比未结合的供体-受体对快21倍。这些结果表明可利用金属增强的FRET来测量大型生物分子的接近程度或用于基于能量转移的分析。
