Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Daru. 2019 Jun;27(1):329-339. doi: 10.1007/s40199-019-00277-0. Epub 2019 May 27.
The use of phytochemicals to prevent or suppress tumours is known as chemoprevention. Numerous plant-derived agents have been reported to have anticancer potentials. As one such anticancer phytochemical, diosgenin has several applications which are nevertheless limited due to its low solubility in water.
We loaded diosgenin into niosome to increase its solubility and hence efficiency. Diosgenin-niosome (diosgenin loaded into niosome) was prepared by thin-film hydration method and characterised by optical microscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), and UV-visible spectrophotometry. Also, loading efficiency, in vitro drug release, and cytotoxicity assay were performed on HepG2 cell line.
Diosgenin-niosome has a nanometric size with a normal size distribution and spherical morphology. The loading efficiency of diosgenin was about 89% with a sustainable and controllable release rate. Finally, the viability of free diosgenin was 61.25%, and after loading into niosomes, it was improved to 28.32%.
The results demonstrated that niosomes increase the solubility of naturally derived hydrophobic chemicals and thus enhance their anticancer effect. Graphical abstract.
利用植物化学物质预防或抑制肿瘤的方法被称为化学预防。已经有许多植物来源的药物被报道具有抗癌潜力。作为一种抗癌植物化学物质，薯蓣皂苷元由于其在水中的低溶解度，其应用受到限制。
我们将薯蓣皂苷元载入脂囊（niosome）以增加其溶解度和效率。采用薄膜水化法制备薯蓣皂苷元脂囊（将薯蓣皂苷元载入脂囊），并通过光学显微镜、动态光散射（DLS）、扫描电子显微镜（SEM）和紫外可见分光光度法进行了表征。同时，在 HepG2 细胞系上进行了载药效率、体外药物释放和细胞毒性测定。
薯蓣皂苷元脂囊具有纳米级尺寸、正常的粒径分布和球形形态。薯蓣皂苷元的载药效率约为 89%，具有可持续和可控的释放速率。游离薯蓣皂苷元的存活率为 61.25%，载入脂囊后提高到 28.32%。
结果表明，脂囊可以提高天然来源的疏水性化学物质的溶解度，从而增强其抗癌作用。
