Teymouri Fatemeh, Karimi Ehsan
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Discov Oncol. 2024 Apr 3;15(1):100. doi: 10.1007/s12672-024-00957-7.
Diosgenin as a potential phytoconstituent and steroidal saponin manifested significant anticancer agents against various cancers. To enhance its solubility and bioavailability in cancer treatment, we loaded diosgenin (PubChem CID: 99474) in poly(lactic-co-glycolide) (PLGA) nanoparticle coated with folic acid-chitosan (Da-PFC-NPs). The diosgenin nano-formulation was characterized and its antioxidant and anticancer properties were surveyed respectively. The obtained results illustrated that the Da-PFC-NPs were spherical and stable with a size of 218 nm and a polydispersity index of 0.41. The Da-PFC-NPs indicated potential free radical scavenging using ABTS and DPPH assay. Meanwhile, it demonstrated selective toxicity against the TUBO breast cancer cell with IC values of 104.45 μg/ml and did not show toxicity on normal cells (I929 cell line). The invivo funding exhibited that Da-PFC-NPs notably altered the liver enzymes (AST, ALT, ALP) and immunoglobulins (IgA, IgG, IgM). Besides that, different doses of Da-PFC-NPs (50 and 100 mg/kg) remarkedly enhance the expression of caspase 3 and decrease HER2 genes. In light of this experiment, we can conclude that Da-PFC-NPs have promise as novel carrier for improving the delivery of diosgenin in cancer therapy.
薯蓣皂苷元作为一种潜在的植物成分和甾体皂苷，是针对多种癌症的重要抗癌剂。为提高其在癌症治疗中的溶解度和生物利用度，我们将薯蓣皂苷元（PubChem CID：99474）负载于包覆有叶酸-壳聚糖的聚乳酸-羟基乙酸共聚物（PLGA）纳米颗粒（Da-PFC-NPs）中。对薯蓣皂苷元纳米制剂进行了表征，并分别研究了其抗氧化和抗癌特性。所得结果表明，Da-PFC-NPs呈球形且稳定，粒径为218 nm，多分散指数为0.41。通过ABTS和DPPH测定法，Da-PFC-NPs显示出潜在的自由基清除能力。同时，它对TUBO乳腺癌细胞表现出选择性毒性，IC值为104.45 μg/ml，对正常细胞（I929细胞系）未显示毒性。体内研究表明，Da-PFC-NPs显著改变了肝酶（AST、ALT、ALP）和免疫球蛋白（IgA、IgG、IgM）。此外，不同剂量的Da-PFC-NPs（50和100 mg/kg）显著提高了caspase 3的表达并降低了HER2基因。鉴于该实验，我们可以得出结论，Da-PFC-NPs有望作为新型载体改善薯蓣皂苷元在癌症治疗中的递送。
