Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Daru. 2019 Jun;27(1):233-241. doi: 10.1007/s40199-019-00265-4. Epub 2019 May 27.
Cisplatin, one of the most effective anticancer drugs, is known to cause undesirable adverse effects, including immunotoxicity. Echinacea purpurea is an important medicinal plant with immunostimulatory and anti-inflammatory activities. We have investigated the protective effect of an herbal formulation (Immulant) containing E. purpurea extract against cisplatin-induced immunotoxicity in rats.
Forty mature albino rats were randomized into four groups (10 rats/group). Control (group 1) animals were subjected to intraperitoneal (i.p.) injection of saline solution (0.2 ml) once every 3 days. Group 2 animals received cisplatin (3.5 mg/kg, i.p.) once every 3 days for successive 2 weeks. Group 3 rats received oral Immulant (150 mg/kg) once daily for 2 weeks. Group 4 animals received oral Immulant treatment as in group 3 in addition to cisplatin as in group 2. Serum level of total protein and albumin, total and differential leukocytic count, phagocytic activity of monocytes, humoral activity and splenic histopathology and immunohistochemistry were used as diagnostic markers of immunotoxicity.
Cisplatin induced marked inhibition of cellular immunity as exhibited by significant decrease of leukocytic count, lymphocyte percentage and phagocytic activity with marked increase in neutrophil percentage. Humoral immunity represented by marked inhibition in total protein and γ-globulin concentration and significant inhibition in antibody titer against Mycoplasma gallisepticum were recorded. Histopathological and immunohistochemical observation of the spleen of cisplatin-treated rats revealed obvious pathological findings of marked depletion and degeneration of lymphoid tissue. Co-oral administration of Immulant resulted in substantial improvement of various immunotoxicological indices compared to cisplatin control.
The herbal medicine Immulant is an immunostimulant which could be used to treat the immunotoxic effects of cisplatin. Graphical abstract Cisplatin (CP) is a highly effective antineoplastic DNA alkylating agent. CP induces free radical production causing an oxidative damage.Cisplatin induced marked inhibition in cellular and humoral immunityEchinacea purpurea (Immulant) is a powerful anticytotoxic agent against cisplatin toxicity.
顺铂是最有效的抗癌药物之一，已知其会引起不良的不良反应，包括免疫毒性。紫锥菊是一种具有免疫刺激和抗炎活性的重要药用植物。我们研究了含有紫锥菊提取物的草药配方（Immulant）对大鼠顺铂诱导的免疫毒性的保护作用。
将 40 只成熟白化大鼠随机分为四组（每组 10 只）。对照组（第 1 组）动物接受腹腔（i.p.）注射生理盐水（0.2ml），每 3 天一次。第 2 组动物每 3 天接受 i.p.注射顺铂（3.5mg/kg），连续 2 周。第 3 组大鼠每天接受 150mg/kg 的口服 Immulant，连续 2 周。第 4 组动物接受与第 2 组相同的顺铂治疗，以及与第 3 组相同的口服 Immulant 治疗。血清总蛋白和白蛋白水平、总白细胞和分类白细胞计数、单核细胞吞噬活性、体液免疫和脾组织病理学和免疫组织化学被用作免疫毒性的诊断标志物。
顺铂诱导明显的细胞免疫抑制，表现为白细胞计数、淋巴细胞百分比和吞噬活性显著降低，中性粒细胞百分比显著增加。体液免疫表现为总蛋白和 γ 球蛋白浓度显著抑制，对鸡败血支原体抗体滴度显著抑制。顺铂处理大鼠脾的组织病理学和免疫组织化学观察显示出明显的淋巴组织明显耗竭和变性的病理发现。与顺铂对照组相比，同时口服 Immulant 可显著改善各种免疫毒性指标。
草药 Immulant 是一种免疫刺激剂，可用于治疗顺铂的免疫毒性作用。
