Bonfa E, Chu J L, Brot N, Elkon K B
Clin Immunol Immunopathol. 1987 Oct;45(1):129-38. doi: 10.1016/0090-1229(87)90119-x.
A 22-amino acid synthetic peptide (P peptide) containing the conserved, shared autoantigenic determinants of the ribosomal P proteins was conjugated to rabbit serum albumin and used to analyze anti-P heterogeneity in 17 lupus sera. Anti-P peptide antibodies demonstrated moderate restriction in isotype (IgM and IgG, but not IgA), subclass (predominantly IgG1 and IgG2), light chain type (predominantly kappa) and spectrotype. In one serum, almost exclusive use of IgG2 and the kappa light chain was observed. These findings indicate that there is a nonrandom selection of heavy and light chain constant region genes as well as limited variable region diversity in the anti-P peptide response.
一种含有核糖体P蛋白保守的、共同自身抗原决定簇的22个氨基酸的合成肽（P肽）与兔血清白蛋白偶联，用于分析17份狼疮血清中的抗P异质性。抗P肽抗体在免疫球蛋白类型（IgM和IgG，但不是IgA）、亚类（主要是IgG1和IgG2）、轻链类型（主要是κ链）和光谱类型方面表现出适度的限制性。在一份血清中，观察到几乎 exclusively使用IgG2和κ轻链。这些发现表明，在抗P肽反应中存在重链和轻链恒定区基因的非随机选择以及有限的可变区多样性。 （注：原文中“almost exclusive use”翻译为“几乎 exclusively使用”，这里“exclusively”未翻译完整，可能是原文有误，推测应为“几乎完全使用” ）
