Gharavi A E, Harris E N, Lockshin M D, Hughes G R, Elkon K B
Hospital for Special Surgery, Cornell University Medical Center, New York, NY 10021.
Ann Rheum Dis. 1988 Apr;47(4):286-90. doi: 10.1136/ard.47.4.286.
The IgG subclass and light chain distribution of anticardiolipin and anti-DNA antibodies were determined in serum samples from patients with systemic lupus erythematosus. With an enzyme linked immunosorbent assay (ELISA) and mouse monoclonal antibodies to individual subclasses, significant differences in the distributions of IgG2, IgG3, and IgG4 subclasses were observed between anticardiolipin and anti-DNA antibodies. Whereas anti-DNA antibodies were predominantly IgG1 and IgG3, all subclasses of anticardiolipin were detected with a prevalence ranging from 34% (IgG3) to 57% (IgG1). Clinical complications were found slightly more frequently (83%) in patients with sera containing the non or weak complement fixing subclasses (IgG2 and IgG4) than in patients with sera containing complement fixing (IgG1 and IgG3) subclasses (62%). Light chain analysis by ELISA showed a trend towards use of kappa chains for anti-DNA and lambda chains for anticardiolipin antibodies. These findings further emphasise the differences between anti-DNA and anticardiolipin antibodies in terms of their origins and potential mechanisms for producing tissue injury.
在系统性红斑狼疮患者的血清样本中，测定了抗心磷脂抗体和抗DNA抗体的IgG亚类及轻链分布情况。采用酶联免疫吸附测定法（ELISA）以及针对各个亚类的小鼠单克隆抗体，观察到抗心磷脂抗体和抗DNA抗体在IgG2、IgG3和IgG4亚类的分布上存在显著差异。抗DNA抗体主要为IgG1和IgG3，而抗心磷脂抗体的所有亚类均被检测到，其流行率在34%（IgG3）至57%（IgG1）之间。与含有补体结合亚类（IgG1和IgG3）的血清患者相比，含有非补体结合或弱补体结合亚类（IgG2和IgG4）血清的患者临床并发症的发生频率略高（83%对62%）。通过ELISA进行的轻链分析显示，抗DNA抗体倾向于使用κ链，而抗心磷脂抗体倾向于使用λ链。这些发现进一步强调了抗DNA抗体和抗心磷脂抗体在起源及产生组织损伤的潜在机制方面的差异。
