Kakumu S, Fuji A, Ohtani Y, Murase K, Hirofuji H, Tahara H
Gastroenterol Jpn. 1987 Jun;22(3):337-43. doi: 10.1007/BF02774260.
Proliferation and IgG synthesis of peripheral blood mononuclear cells (PBMC) in response to stimulation with recombinant interleukin 2 (IL-2) and Staphylococcus aureus Cowan 1 (SAC) were evaluated in 32 patients with chronic persistent hepatitis (CPH), chronic active hepatitis (CAH) and liver cirrhosis (LC). Eleven patients had serum HBe antigen, 10 presented with HBe antibody and 11 had non-A, non-B hepatitis. IgG synthesis of PBMC induced with the two stimuli was significantly decreased in patients with CAH and LC when compared with that of controls. However, the generated amounts of IgG were not associated with the HB virus carrier state. B cells and T4+ cells were responsible for the diminished IgG synthesis in patients with CAH and LC when assessed by coculture experiments. On the other hand, proliferative response of PBMC to IL-2 and SAC were similar in controls and patient groups. These findings indicate that IgG production level of PBMC stimulated with IL-2 and SAC can reflect the severity of the underlying disease in chronic hepatitis patients.
在32例慢性持续性肝炎（CPH）、慢性活动性肝炎（CAH）和肝硬化（LC）患者中，评估了外周血单核细胞（PBMC）对重组白细胞介素2（IL-2）和金黄色葡萄球菌Cowan 1（SAC）刺激的增殖和IgG合成情况。11例患者血清HBe抗原阳性，10例呈现HBe抗体，11例患有非甲非乙型肝炎。与对照组相比，CAH和LC患者中由这两种刺激诱导的PBMC的IgG合成显著降低。然而，IgG的生成量与HB病毒携带状态无关。通过共培养实验评估时，B细胞和T4 +细胞是导致CAH和LC患者IgG合成减少的原因。另一方面，对照组和患者组中PBMC对IL-2和SAC的增殖反应相似。这些发现表明，用IL-2和SAC刺激的PBMC的IgG产生水平可以反映慢性肝炎患者潜在疾病的严重程度。
