大鼠的免疫球蛋白λ基因座由两个Cλ基因和一个单一的Vλ基因组成。

The immunoglobulin lambda locus in rat consists of two C lambda genes and a single V lambda gene.

作者信息

Steen M L, Hellman L, Pettersson U

出版信息

Gene. 1987;55(1):75-84. doi: 10.1016/0378-1119(87)90250-2.

Abstract

The immunoglobin lambda locus of the rat has been studied. Germ-line V lambda and C lambda genes were isolated from recombinant-phage libraries and characterized by nucleotide sequencing. The results showed that the lambda locus of the rat contains one single V lambda gene and two C lambda genes, thus representing one of the least complex lambda loci so far characterized. The two C lambda genes are separated by a spacer approx. 3 kb long. Two J segments are located at the 5' side of each C lambda gene. One of the C lambda genes (C lambda 1) probably represents a pseudogene, as the J lambda 1 segments have non-functional recombination and splice signals. The organization of the rat lambda locus resembles that of mouse, except that only one cluster is present in the rat. Thus since the evolutionary separation of the rat and mouse species ten MYR ( = 10(6) years) ago, either one cluster has been lost from the rat, or duplicated in the mouse.

摘要

对大鼠的免疫球蛋白λ基因座进行了研究。从重组噬菌体文库中分离出种系Vλ和Cλ基因，并通过核苷酸测序进行了表征。结果表明，大鼠的λ基因座包含一个单一的Vλ基因和两个Cλ基因，因此是迄今为止所表征的最不复杂的λ基因座之一。两个Cλ基因被一个约3kb长的间隔区隔开。两个J片段位于每个Cλ基因的5'端。其中一个Cλ基因（Cλ1）可能代表一个假基因，因为Jλ1片段具有无功能的重组和剪接信号。大鼠λ基因座的组织与小鼠相似，只是大鼠中只存在一个簇。因此，自从大鼠和小鼠物种在1000万年前进化分离以来，要么大鼠失去了一个簇，要么小鼠中发生了复制。