Blomberg B, Traunecker A, Eisen H, Tonegawa S
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3765-9. doi: 10.1073/pnas.78.6.3765.
We have cloned four lambda light chain constant region (C) genes from mouse embryo DNA. Each carries its own joining (J) segment approximately 1.3 kilobases to its 5' side. The four C genes occur in two clusters, 5' J3C3J1C13' and 5' J2C2J4C43', with C4 being a new C lambda gene. We have also shown that V lambda 1 is joined productively with C lambda 3 in a lambda 3-producing myeloma, and it is most likely that V lambda 1 and V lambda 2 are the only V lambda genes. Based on the analysis of the germ line and rearranged variable region (V) lambda genes in myelomas we argue that the V lambda 1 and V lambda 2 genes are at the 5' side of the C3C1 and C2C4 clusters, respectively. We propose that the two clusters arose by duplication. We also speculate on the role of J-associated DNA sequences in regulation of expression of the lambda subtypes.
我们从小鼠胚胎DNA中克隆出了四个λ轻链恒定区（C）基因。每个基因在其5'端约1.3千碱基处都带有自身的连接（J）片段。这四个C基因以两个簇的形式出现，即5' J3C3J1C1 3'和5' J2C2J4C4 3'，其中C4是一个新的Cλ基因。我们还表明，在产生λ3的骨髓瘤中，Vλ1与Cλ3有效连接，并且Vλ1和Vλ2很可能是仅有的Vλ基因。基于对骨髓瘤中种系和重排可变区（V）λ基因的分析，我们认为Vλ1和Vλ2基因分别位于C3C1和C2C4簇的5'端。我们提出这两个簇是通过复制产生的。我们还推测了J相关DNA序列在λ亚型表达调控中的作用。
