Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
Division of Oral Medicine and Dentistry, Brigham and Women's Hospital, Boston, Massachusetts.
Oral Dis. 2019 Jun;25 Suppl 1:12-27. doi: 10.1111/odi.13063.
Advances in high-throughput sequencing technologies have allowed for a rapid increase in knowledge about the human microbiome in both healthy and diseased states, which is expected to increase our understanding of multifactorial diseases. The World Workshop on Oral Medicine VII chose the microbiome as one of its topics of focus. Part 1 of this review provides updated knowledge in the field of microbiome research, describes the advantages and disadvantages of currently available sequencing technologies, and proposes a seven-step "recipe" for designing and performing studies that is supported by contemporary evidence. Part 2 of this review in a companion paper discusses the results of high-throughput sequencing studies published to date on the microbiota associated with oral mucosal diseases. The goal of this collective enterprise is to encourage more oral medicine specialists to become engaged in multidisciplinary collaborations to investigate the role of the microbiome in relation to oral diseases, which could potentially lead to enhanced diagnosis, risk assessment and treatment of these patients.
高通量测序技术的进步使人们对健康和疾病状态下的人类微生物组有了快速的了解，这有望提高我们对多因素疾病的认识。第七届世界口腔医学研讨会选择微生物组作为其关注的主题之一。本文第一部分提供了微生物组研究领域的最新知识，描述了目前可用的测序技术的优缺点，并提出了一个由当代证据支持的设计和进行研究的七步“配方”。本文第二部分将在另一篇论文中讨论迄今为止发表的关于与口腔黏膜疾病相关的微生物组的高通量测序研究结果。这项集体事业的目标是鼓励更多的口腔医学专家参与多学科合作，以研究微生物组在口腔疾病中的作用，这可能会提高对这些患者的诊断、风险评估和治疗水平。
