Buczek Alicja, Bartosik Katarzyna, Buczek Weronika, Buczek Alicja M, Kuczyński Paweł
Chair and Department of Biology and Parasitology, Medical University of Lublin, Radziwiłłowska 11 St., 20-080, Lublin, Poland.
Chair and Department of Rehabilitation and Orthopaedics, Medical University of Lublin, Jaczewskiego 8 St, 20-090, Lublin, Poland.
Exp Appl Acarol. 2019 Jun;78(2):203-221. doi: 10.1007/s10493-019-00381-5. Epub 2019 May 29.
Two pyrethroids, deltamethrin (D) and alphacypermethrin (AC), have been used as commercial products for tick control worldwide. However, the effects of sublethal doses of these compounds on various tick species and their developmental stages have not been fully explored yet, although such knowledge could contribute to a more effective application of both pyrethroids and simultaneous reduction of their costs and undesirable side effects, including the harmful impact on other organisms and environmental contamination. This study investigates the effect of sublethal concentrations of D and AC applied to engorged females on the fecundity, development of eggs and larvae, and the number of offspring in the Ixodes ricinus (L.) tick, which is the most important vector of tick-borne diseases of humans in Europe. After detachment from rabbit's skin, fully engorged I. ricinus females were treated with 20 μl of pyrethroid solutions at five concentrations from 0.01562 to 0.25% and kept at 28 °C and 75% relative humidity. The impact of the pyrethroids on maturation and development of eggs as well as development of larvae was assessed based on parameters of the preoviposition and oviposition periods and the course of embryogenesis and egg hatch. The investigations have shown that both tested acaricides reduce the reproductive rate in I. ricinus females by inhibition of egg development and disturb embryonic development and larval hatch. Females did not lay eggs at concentrations higher than 0.0625% AC and 0.125% D. The lower concentrations of both pyrethroids disturbed or inhibited the embryogenesis and egg hatch in I. ricinus.
两种拟除虫菊酯，溴氰菊酯（D）和高效氯氰菊酯（AC），已作为全球控制蜱虫的商业产品使用。然而，尽管此类知识有助于更有效地应用这两种拟除虫菊酯，并同时降低其成本和不良副作用，包括对其他生物的有害影响和环境污染，但这些化合物的亚致死剂量对各种蜱虫物种及其发育阶段的影响尚未得到充分研究。本研究调查了将D和AC的亚致死浓度应用于饱血雌蜱后，对蓖麻硬蜱（Ixodes ricinus (L.)）的繁殖力、卵和幼虫发育以及后代数量的影响，蓖麻硬蜱是欧洲人类蜱传疾病最重要的传播媒介。从兔皮上脱离后，用20微升浓度从0.01562%至0.25%的五种拟除虫菊酯溶液处理完全饱血的蓖麻硬蜱雌蜱，并将其置于28°C和75%相对湿度的环境中。根据产卵前期和产卵期参数以及胚胎发生和卵孵化过程，评估拟除虫菊酯对卵的成熟和发育以及幼虫发育的影响。研究表明，两种测试的杀螨剂均通过抑制卵发育降低了蓖麻硬蜱雌蜱的繁殖率，并干扰了胚胎发育和幼虫孵化。当AC浓度高于0.0625%和D浓度高于0.125%时，雌蜱不产卵。两种拟除虫菊酯的较低浓度均干扰或抑制了蓖麻硬蜱的胚胎发生和卵孵化。
