Yokomizo Akiyo, Morimoto Yuya, Nishimura Keigo, Takeuchi Shoji
Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Micromachines (Basel). 2019 May 29;10(6):358. doi: 10.3390/mi10060358.
In this paper, we propose an anchoring device with pillars to immobilize an adipocyte microfiber that has a fiber-shaped adipocyte tissue covered by an alginate gel shell. Because the device enabled the immobilization of the microfiber in a culture dish even after its transportation and the exchange of the culture medium, we can easily track the specific positions of the microfiber for a long period. Owing to the characteristics of the anchoring device, we successfully performed temporal observations of the microfiber on the device for a month to investigate the function and morphology of three-dimensional cultured adipocytes. Furthermore, to demonstrate the applicability of the anchoring device to drug testing, we evaluated the lipolysis of the microfiber's adipocytes by applying reagents with an anti-obesity effect. Therefore, we believe that the anchoring device with the microfiber will be a useful tool for temporal biochemical analyses.
在本文中，我们提出了一种带有支柱的锚定装置，用于固定脂肪细胞微纤维，该微纤维具有由藻酸盐凝胶壳覆盖的纤维状脂肪组织。由于该装置即使在运输和更换培养基后仍能将微纤维固定在培养皿中，我们可以轻松地长期追踪微纤维的特定位置。由于锚定装置的特性，我们成功地在该装置上对微纤维进行了为期一个月的时间观察，以研究三维培养脂肪细胞的功能和形态。此外，为了证明锚定装置在药物测试中的适用性，我们通过应用具有抗肥胖作用的试剂来评估微纤维脂肪细胞的脂解作用。因此，我们认为带有微纤维的锚定装置将成为进行时间生化分析的有用工具。
