Department of Medicinal and Aromatic Plant, High Altitude Plant Physiology Research Centre, Hemvati Nandan Bahuguna Garhwal (Central) University, Srinagar Garhwal, Uttarakhand 246174, India.
Department of Chemistry, Hemvati Nandan Bahuguna Garhwal (Central) University, Srinagar Garhwal, Uttarakhand 246174, India.
Mater Sci Eng C Mater Biol Appl. 2019 Sep;102:212-220. doi: 10.1016/j.msec.2019.04.035. Epub 2019 Apr 13.
The field of nanoscience or nanotechnology is gaining popularity day by day and it is now well established field of interdisciplinary sciences. Nanoparticles are synthesized by various methods but suffer from few limitations such as toxicity. To overcome the limitations of various synthesis methods, the focus has shifted to new radius which involves biological organisms such as plants i.e. green synthesis. This phyto-mediated approach using various plant parts and extracts thereof, is considered to be new, simple and cheaper strategy to synthesize metal nanoparticles. In the present work, we describe a simple, eco-friendly and low-cost procedure for biosynthesis of zinc oxide nanoparticles (ZnO NPs) using leaf extract of medicinally important plant, Berberis aristata. The synthesized zinc oxide nanoparticles was subsequently characterized by using UV-Visible spectroscopy, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Analysis (EDX) and Dynamic Light Scattering (DLS) which proved the synthesized ZnO NPs to fall in the range of nanoparticles. Synthesized ZnO NPs were further characterized and screened for antimicrobial and antioxidant activity. ZnO NPs displayed antibacterial activities against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Bacillus subtilis, Bacillus cereus, Serratia marcescens but not for Proteus and Salmonella Typhi. ZnO NPs displayed antibacterial activities and moderate antioxidant potential. Our results display the synthesized ZnO NPs to fall in an important class of potential antibacterial, antioxidant agents and effective against urinary tract infections.
纳米科学或纳米技术领域日益受到关注，现已成为跨学科科学领域的重要分支。纳米粒子可以通过多种方法合成，但存在一些局限性，如毒性。为了克服各种合成方法的局限性，研究重点已经转移到新的领域，涉及生物有机体，如植物，即绿色合成。这种使用各种植物部分及其提取物的植物介导方法被认为是一种新的、简单和更经济的策略，用于合成金属纳米粒子。在本工作中，我们描述了一种使用药用植物小檗的叶提取物来生物合成氧化锌纳米粒子（ZnO NPs）的简单、环保和低成本方法。使用紫外-可见分光光度法、X 射线衍射（XRD）、扫描电子显微镜（SEM）、能谱分析（EDX）和动态光散射（DLS）对合成的氧化锌纳米粒子进行了表征，证明所合成的 ZnO NPs 属于纳米粒子范围。进一步对合成的 ZnO NPs 进行了表征，并对其抗菌和抗氧化活性进行了筛选。ZnO NPs 对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌、枯草芽孢杆菌、蜡状芽孢杆菌、粘质沙雷氏菌具有抗菌活性，但对变形菌和伤寒沙门氏菌没有活性。ZnO NPs 具有抗菌活性和适度的抗氧化潜力。我们的结果表明，所合成的 ZnO NPs 属于一类有潜力的抗菌、抗氧化剂，可有效治疗尿路感染。
