Hussain Amjad, Azam Sajjad, Rehman Kanwal, Ali Meher, Hamid Akash Muhammad Sajid, Zhou Xuefeng, Rauf Abdur, Alshammari Abdulrahman, Albekairi Norah A, Al-Ghamdi Abdullah Hamed, Quresh Ahmad Kaleem, Khan Shoaib, Khan Muhammad Usman
Institute of Chemistry, University of Okara, Okara, 56300, Punjab, Pakistan.
Department of Pharmacy, The Women University, Multan, Pakistan.
Heliyon. 2024 Jun 22;10(13):e33327. doi: 10.1016/j.heliyon.2024.e33327. eCollection 2024 Jul 15.
This investigation portrays the phytochemical screening, green synthesis, characterization of Fe and Zn nanoparticles, their antibacterial, anti-inflammation, cytotoxicity, and anti-thrombolytic activities. Four dissimilar solvents such as, -hexane, chloroform, ethyl acetate and -butanol were used to prepare the extracts of Royle ex Benth. This is valued medicinal plant (Family Lamiaceae), native to mountains of Afghanistan and Kashmir. In the GC-MS study of its extract, the identified phytoconstituents have different nature such as terpenoids, alcohol and esters. The synthesized nanoparticles were characterized by SEM, UV, XRD, and FT-IR. The phytochemical analysis showed that the plant contains TPC (total phenolic content) 297.51 mg GAE/g and TFC (total flavonoid content) 467.24 mg CE/g. The cytotoxicity values have shown that the chloroform, -butanol and aqueous extracts were more toxic than other extracts. The anti-inflammatory potential of -butanol and aqueous extracts was found higher than all other extracts. Chloroform and -hexane extracts have low MIC values against both and bacterial strains. Chloroform and aqueous extracts have great anti-thrombolytic potential than all other extracts. Overall, this study successfully synthesized the nanoparticles and provides evidence that have promising bioactive compounds that could serve as potential source in the drug formulation.
本研究描述了植物化学筛选、铁和锌纳米颗粒的绿色合成、表征及其抗菌、抗炎、细胞毒性和抗血栓溶解活性。使用四种不同的溶剂，如正己烷、氯仿、乙酸乙酯和正丁醇，制备了皱叶留兰香（Mentha crispa Royle ex Benth.）的提取物。这是一种珍贵的药用植物（唇形科），原产于阿富汗和克什米尔的山区。在其提取物的气相色谱-质谱联用（GC-MS）研究中，鉴定出的植物成分具有不同的性质，如萜类、醇类和酯类。通过扫描电子显微镜（SEM）、紫外可见光谱（UV）、X射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）对合成的纳米颗粒进行了表征。植物化学分析表明，该植物含有总酚含量（TPC）297.51 mg没食子酸当量/g和总黄酮含量（TFC）467.24 mg芦丁当量/g。细胞毒性值表明，氯仿、正丁醇和水提取物比其他提取物毒性更大。发现正丁醇和水提取物的抗炎潜力高于所有其他提取物。氯仿和正己烷提取物对两种细菌菌株的最低抑菌浓度（MIC）值较低。氯仿和水提取物的抗血栓溶解潜力比所有其他提取物都大。总体而言，本研究成功合成了纳米颗粒，并提供了证据表明皱叶留兰香含有有前景的生物活性化合物，可作为药物制剂的潜在来源。
