Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, College of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang, 473004, People's Republic of China.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Extremophiles. 2019 Sep;23(5):521-528. doi: 10.1007/s00792-019-01104-9. Epub 2019 May 30.
Two extreme halophilic archaeal strains, SYSUA9-0 and SYSUA9-1, were isolated from Ebi lake of Xinjiang, China. The colonies were Gram-negative, coccoid, and non-motile. Strains were aerobic and grew at 25-50 °C (optimum at 37 °C), in the presence of 10-35% (w/v) NaCl (optimum at 20-22%), and pH 6.0-8.0 (optimum at 7.0). The 16S rRNA gene sequence result revealed that the two strains were closely related to Haloprofundus marisrubri SB9 (92.7% similarity). The DNA-DNA hybridization value (97% ± 1%) suggested that SYSUA9-0 and SYSUA9-1 were similar; however, their sequence similarities with other archaeal members suggested that they were novel candidates. The genomic G + C content of SYSUA9-0 was 66.9%. The average nucleotide identity value between SYSU A9-0 and Haloprofundus marisrubri SB9 was 69.1%, which was far below the cutoff value (95-96%) proposed to define the species boundary. The polar lipids were phosphatidylglycerol (PG), phosphatidylglycerolphosphate methylester (PGP-Me), sulfated mannosyl glucosyl diether, mannosyl glucosyldiether, and four unidentified glycolipids. Phenotypic, chemotaxonomic and comparative genome analysis suggested that SYSU A9-0 and SYSU A9-1 represent a novel species of a new genus within the family Haloferacaceae, for which the name Halegenticoccus soli gen. nov., sp. nov., is proposed. The type strain is SYAUA9-0 (= KCTC4241 = CGMCC 1.15765).
两株极端嗜盐古菌菌株 SYSUA9-0 和 SYSUA9-1 从中国新疆艾比湖分离得到。菌落革兰氏阴性，球形，无运动性。菌株为需氧菌，在 25-50°C（最佳温度为 37°C）、10-35%（w/v）NaCl（最佳浓度为 20-22%）和 pH6.0-8.0（最佳 pH 值为 7.0）条件下生长。16S rRNA 基因序列结果表明，这两株菌与深海盐红菌 SB9 （相似度 92.7%）密切相关。DNA-DNA 杂交值（97%±1%）表明 SYSUA9-0 和 SYSUA9-1 相似，但与其他古菌成员的序列相似性表明它们是新的候选种。SYSUA9-0 的基因组 G+C 含量为 66.9%。SYSU A9-0 与深海盐红菌 SB9 的平均核苷酸同一性值为 69.1%，远低于定义种间边界的 95-96%的建议值。极性脂类为磷脂酰甘油（PG）、磷脂酰甘油磷酸甲醚（PGP-Me）、硫酸甘露糖葡糖基二醚、甘露糖葡糖基二醚和四种未鉴定的糖脂。表型、化学分类和比较基因组分析表明，SYSU A9-0 和 SYSU A9-1 代表了 Haloferacaceae 科一个新属的新种，建议将其命名为 Solihalegenticoccus soli gen. nov.，sp. nov.。模式菌株为 SYAUA9-0（=KCTC4241=CGMCC 1.15765）。
