Gidlund M, Ojo E A, Orn A, Wigzell H, Murgita R A
Scand J Immunol. 1979;9(2):167-73. doi: 10.1111/j.1365-3083.1979.tb02719.x.
Mice were treated with a heterologous anti-IgM serum to obtain B-cell-deprived mice. Spleen cells from normal and B-cell-deprived mice were tested in three different cytolytic systems: natural killer cells (NK); antibody-dependent cell-mediated cytolysis (ADCC) against an NK-sensitive tumour, P815; and ADCC against chicken erythrocytes. The impact of administration of an interferon-inducing NK enhancing agent, Tilorone, was also investigated. Whereas the cell population from B-cell-deprived mice was significantly suppressed in antibody-producing cells, the capacity to function in NK or ADCC was largely unimpaired both before and after administration of Tilorone. Our results would imply that mature B cells play no significant role in either the maturation of the NK cells or the expression of their cytolytic ability. Furthermore, effector cells for both NK and ADCC against antibody-coated tumour target cells were found to be distinct from those functioning in ADCC against chicken erythrocytes.
用异种抗IgM血清处理小鼠以获得B细胞缺失小鼠。在三种不同的细胞溶解系统中测试正常小鼠和B细胞缺失小鼠的脾细胞：自然杀伤细胞（NK）；针对NK敏感肿瘤P815的抗体依赖性细胞介导的细胞溶解（ADCC）；以及针对鸡红细胞的ADCC。还研究了给予干扰素诱导的NK增强剂泰洛龙的影响。虽然B细胞缺失小鼠的细胞群体在抗体产生细胞中受到显著抑制，但在给予泰洛龙前后，其在NK或ADCC中发挥功能的能力基本未受损害。我们的结果表明，成熟B细胞在NK细胞的成熟或其细胞溶解能力的表达中均不发挥重要作用。此外，发现针对抗体包被肿瘤靶细胞的NK和ADCC效应细胞与针对鸡红细胞的ADCC中发挥作用的细胞不同。
