Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Bioresour Technol. 2019 Sep;288:121559. doi: 10.1016/j.biortech.2019.121559. Epub 2019 May 25.
To investigate the feasibility of simultaneously enhancing lipid and carotene production by irradiation with different carbon sources, a strategy by controlling the carbon sources supply were selected to culture Rhodotorula glutinis under the irradiation condition. The results demonstrated that the irradiation indeed enhanced cell growth, lipid and carotene production with different carbon sources supply. Besides, the fatty acids profiling as revealed by more unsaturated fatty acids (mainly C16:1, C18:2 and C18:3) and less saturated fatty acids (C18:0, C22:0 and C24:0) were found during the process of irradiation. Compared with the control, the increase of the transcription levels in genes connected with substrates assimilation, lipid production and carotene accumulation were observed under the irradiation condition. The results suggest the possibility of using irradiation as an effective strategy to increase the production of both lipid and carotene with the controlled carbon sources supply.
为了研究利用不同碳源辐照同时提高脂质和类胡萝卜素产量的可行性，选择了一种通过控制碳源供应来培养粘红酵母的策略，在辐照条件下进行培养。结果表明，辐照确实可以在不同碳源供应下增强细胞生长、脂质和类胡萝卜素的产生。此外，在辐照过程中还发现了脂肪酸组成的变化，即更多的不饱和脂肪酸（主要是 C16:1、C18:2 和 C18:3）和较少的饱和脂肪酸（C18:0、C22:0 和 C24:0）。与对照组相比，在辐照条件下，与底物同化、脂质生产和类胡萝卜素积累相关的基因转录水平增加。结果表明，利用辐照作为一种有效的策略，通过控制碳源供应来增加脂质和类胡萝卜素的产量是可能的。
