Yang Jiawen, Lin Donghai, Deng Chuwei, Li Zheng, Pu Yan, Yu Yanfang, Li Kai, Li Ding, Chen Peng, Chen Feng
Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China.
School of Medicine, Southeast University, Nanjing, Jiangsu 210009, PR China.
Forensic Sci Int. 2019 Aug;301:101-106. doi: 10.1016/j.forsciint.2019.05.024. Epub 2019 May 20.
In forensic genetics, the analysis of DNA in biological samples is a valuable tool for personal identification. There is an increasing demand in analyzing of the mixed DNA which may provide insightful investigative instructions. With the continuous effort for the improvement of individual identification, complicated mixed stains represent a growing fraction of the samples processed by forensic laboratories. Recent technological advances have enabled quantitative analysis of DNA mixture and emerging sequencing approaches to decipher the complicated DNA mixture. Here, we describe the use of different genetic markers, typing approaches and analytical methods in mixture analysis, and how useful information can be obtained from complicated DNA mixture.
在法医遗传学中，对生物样本中的DNA进行分析是个人身份鉴定的一项重要工具。对混合DNA进行分析的需求日益增加，因为它可能提供有价值的调查线索。随着个体识别技术的不断改进，复杂的混合污渍在法医实验室处理的样本中所占比例越来越大。最近的技术进步使得对DNA混合物进行定量分析成为可能，并且出现了用于破解复杂DNA混合物的测序方法。在这里，我们描述了在混合物分析中使用不同的遗传标记、分型方法和分析方法，以及如何从复杂的DNA混合物中获得有用信息。
