Maudgal P C, Missotten L
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1979 Feb 8;209(4):239-48. doi: 10.1007/BF00419058.
Three days after herpes simplex virus inoculation, an increased amount of DNA and RNA was observed in the superficial epithelium cells of rabbit cornea. Histochemical staining demonstrated the development of acid mucopolysaccharides and the destruction of reticulin. In the early stages, on rare occasions, giant polykaryocytes with multiple micronuclei were seen. From 1 week after infection, more and more cells became rounded and shrunken. Cytoplasm of these cells might contain DNA diffusely interspersed with RNA. This DNA is probably viral in nature. The nuclei of these cells varied in shape, size, and staining intensity. Nuclear fragments were often observed in the cytoplasm. Stainings for acid mucopolysaccharides were strongly positive in the rounded cells. These cells fused to form syncytia Variable-sized pseudopodialike processes containing DNA and RNA extend from some of the rounded and liquefied cells toward other cells. In the later stages, development of ghost cells was seen. Histochemical methods demonstrated the deposition of acid mucopolysaccharides on their cell membranes. Necrosis was more often present in the late stages. Nuclear debris and deformed cells were encountered in such areas. On the healing of the keratitis, 3 months after inoculation, the cell cytology and staining reactions reverted to normal.
单纯疱疹病毒接种三天后，在兔角膜的浅表上皮细胞中观察到DNA和RNA含量增加。组织化学染色显示酸性粘多糖的形成和网状纤维的破坏。在早期，偶尔可见带有多个微核的巨大多核细胞。感染一周后，越来越多的细胞变得圆形并皱缩。这些细胞的细胞质可能含有弥散分布的DNA并夹杂着RNA。这种DNA可能本质上是病毒性质的。这些细胞的细胞核在形状、大小和染色强度上各不相同。在细胞质中经常观察到核碎片。酸性粘多糖染色在圆形细胞中呈强阳性。这些细胞融合形成多核巨细胞。一些圆形和液化的细胞伸出含有DNA和RNA的大小不一的伪足样突起伸向其他细胞。在后期，可见空泡细胞的形成。组织化学方法显示酸性粘多糖沉积在它们的细胞膜上。坏死在后期更为常见。在这些区域可遇到核碎片和变形细胞。接种三个月后，角膜炎愈合时，细胞细胞学和染色反应恢复正常。
