新型 Ourmia 样病毒感染. 的分子特征
Molecular Characterization of a Novel Ourmia-Like Virus Infecting .
机构信息
Key Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou 570228, China.
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China.
出版信息
Viruses. 2020 Feb 19;12(2):231. doi: 10.3390/v12020231.
Here, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a product of RNA-dependent RNA polymerase (RdRp) by both standard and mitochondrial genetic codons. Cellular fractionation assay indicated that PmOLV1 RNAs are likely more enriched in mitochondria than in cytoplasm. Phylogenetic analysis indicated that PmOLV1 is a new member of the genus (recently proposed) within the family .
这里，我们报告了一种新型的（+）ssRNA 真菌病毒，即来自 LG915-1 菌株的长喙壳伞素类似病毒 1（PmOLV1）。PmOLV1 的基因组长 2603 个核苷酸，包含一个单一的开放阅读框（ORF），该 ORF 可以通过标准和线粒体遗传密码子翻译成 RNA 依赖性 RNA 聚合酶（RdRp）的产物。细胞分离测定表明，PmOLV1 RNA 可能比细胞质更富集在线粒体中。系统发育分析表明，PmOLV1 是新近提出的病毒科 属（）的一个新成员。
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