Robson G D, Bell S D, Kuhn P J, Trinci A P
Department of Cell and Structural Biology, School of Biological Sciences, University of Manchester, UK.
J Gen Microbiol. 1987 Feb;133(2):361-7. doi: 10.1099/00221287-133-2-361.
The growth of colonies of Rhizoctonia cerealis and Penicillium chrysogenum on solid media in plate cultures was studied. When grown on defined media containing 10-50 mM-glucose, R. cerealis did not cause a significant reduction in the glucose concentration of the medium in advance of colonization, but did cause the formation of a steep glucose concentration gradient in the substrate below the colony; the medium directly below the centre of a 7 cm diameter colony of R. cerealis was exhausted of glucose even when the fungus was grown on medium containing 50 mM-glucose. Penicillin produced by colonies of P. chrysogenum accumulated in the medium in advance of fungal colonization. For a period up to about 18 d after inoculation, the concentration of penicillin in the medium throughout the plate increased with colony development and thereafter, except at the margins of the plate, decreased.
研究了禾谷丝核菌和产黄青霉在平板培养固体培养基上菌落的生长情况。当在含有10 - 50 mM葡萄糖的限定培养基上生长时，禾谷丝核菌在定殖之前不会导致培养基中葡萄糖浓度显著降低，但会在菌落下方的底物中形成陡峭的葡萄糖浓度梯度；即使真菌在含有50 mM葡萄糖的培养基上生长，直径7 cm的禾谷丝核菌菌落中心正下方的培养基中的葡萄糖也会耗尽。产黄青霉菌落产生的青霉素在真菌定殖之前就积累在培养基中。在接种后长达约18天的时间内，平板上整个培养基中的青霉素浓度随着菌落的发育而增加，此后，除了平板边缘外，浓度下降。
