a State Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University , Hangzhou , China.
b Department of Crop Protection, Federal University of Agriculture Abeokuta , Abeokuta , Nigeria.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):2230-2239. doi: 10.1080/21691401.2019.1622552.
Bacterial brown stripe (BBS) is one of the most economically important diseases of rice caused by (Ao). In order to ensure food security and safe consumption, the use of non-chemical approach is necessary. In this study, MgO and MnO were synthesized using chamomile flower extract. The synthesized MgO and MnO nanoparticles were characterized by UV-Visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission/scanning electron microscopy. The sizes were 18.2 and 16.5 nm for MgO and MnO nanoparticles, respectively. The MgO and MnO nanoparticles reduced the growth of Ao strain RS-2 by 62.9 and 71.3%, respectively. Also, the biofilm formation and swimming motility were significantly reduced compared to the control. The antibacterial mechanisms of MgO and MnO nanoparticles against RS-2 reveals that MgO and MnO nanoparticles penetrated the cells and destroyed the cell membrane leading to leakage of cytoplasmic content. Also, the flow cytometry observation reveals that the apoptotic cell ratio of RS-2 increased from 0.97% to 99.52 and 99.94% when treated with MgO and MnO nanoparticles, respectively. Altogether, the results suggest that the synthesized MgO and MnO nanoparticles could serve as an alternative approach method for the management of BBS.
细菌棕色条纹（BBS）是由 （Ao）引起的水稻最重要的经济疾病之一。为了确保食品安全和安全消费，有必要采用非化学方法。在这项研究中，使用洋甘菊花提取物合成了 MgO 和 MnO。通过紫外可见光谱、傅里叶变换红外光谱、X 射线衍射、透射/扫描电子显微镜对合成的 MgO 和 MnO 纳米粒子进行了表征。MgO 和 MnO 纳米粒子的尺寸分别为 18.2nm 和 16.5nm。MgO 和 MnO 纳米粒子将 Ao 菌株 RS-2 的生长分别降低了 62.9%和 71.3%。与对照相比，生物膜形成和泳动性也显著降低。MgO 和 MnO 纳米粒子对 RS-2 的抗菌机制表明，MgO 和 MnO 纳米粒子穿透细胞并破坏细胞膜，导致细胞质内容物泄漏。此外，流式细胞术观察表明，当用 MgO 和 MnO 纳米粒子处理时，RS-2 的凋亡细胞比例从 0.97%增加到 99.52%和 99.94%。总之，结果表明，合成的 MgO 和 MnO 纳米粒子可以作为 BBS 管理的替代方法。
