Al-Harbi Laila M, Ezzeldien Mohammed, Elhenawy Ahmed A, Said Alaa Hassan
Department of Chemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Physics Department, College of Science, Jouf University, Sakaka, Saudi Arabia.
Front Bioeng Biotechnol. 2024 Nov 29;12:1480694. doi: 10.3389/fbioe.2024.1480694. eCollection 2024.
(neem) extract was used to biologically synthesize magnesium oxide nanoparticles (MgO NPs). The synthesized NPs were characterized using X-ray diffraction (XRD), thermogravimetric analysis (TGA), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier-transform infrared (FTIR), and UV-vis spectroscopy. Antioxidant, anticancer, antibacterial, antidiabetic, and anti-inflammatory activities were analyzed for the synthesized MgO NPs and neem extract. The obtained results confirmed the synthesis of spherical magnesium oxide nanoparticles with an average particle size of 23 nm using XRD. The samples exhibited good thermal stability and high stability in biological media. Compared to the neem extract and chemically synthesized magnesium oxide nanoparticles, the bioinspired magnesium oxide nanoparticles showed considerable free radical scavenging activity, with an IC value of 69.03 μg/mL. In addition, they reflected high selectivity to liver hepatic cancer cells with an IC value of 94.85 μg/mL without inducing any damage to human umbilical vein endothelial cells. The antibacterial activity of the bioinspired magnesium oxide nanoparticles demonstrated comparable effectiveness in treating both Gram-positive and Gram-negative bacterial strains. Furthermore, the produced bioinspired magnesium oxide nanoparticles showed a high percentage of inhibition for both α-amylase and α-glucosidase enzymes with an IC value of 61. 53 and 50.6 μg/mL, respectively. In addition, the bioinspired magnesium oxide nanoparticles also showed a higher denaturation inhibition percentage with an IC value of 6.66 μg/mL, indicating strong anti-inflammatory action. These enhanced abilities usher in a new bioinspired magnesium oxide nanoparticle bio-application era. Consequently, further studies are needed to assess the kinetic properties of these nanoparticles.
（印楝）提取物被用于生物合成氧化镁纳米颗粒（MgO NPs）。使用X射线衍射（XRD）、热重分析（TGA）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和紫外可见光谱对合成的纳米颗粒进行了表征。对合成的MgO NPs和印楝提取物的抗氧化、抗癌、抗菌、抗糖尿病和抗炎活性进行了分析。所得结果证实了使用XRD合成了平均粒径为23 nm的球形氧化镁纳米颗粒。样品在生物介质中表现出良好的热稳定性和高稳定性。与印楝提取物和化学合成的氧化镁纳米颗粒相比，生物启发合成的氧化镁纳米颗粒表现出相当可观的自由基清除活性，IC值为69.03 μg/mL。此外，它们对肝癌细胞具有高选择性，IC值为94.85 μg/mL，且不会对人脐静脉内皮细胞造成任何损伤。生物启发合成的氧化镁纳米颗粒的抗菌活性在治疗革兰氏阳性和革兰氏阴性细菌菌株方面显示出相当的有效性。此外，所制备的生物启发合成的氧化镁纳米颗粒对α-淀粉酶和α-葡萄糖苷酶的抑制率分别高达61.53和50.6 μg/mL。此外，生物启发合成的氧化镁纳米颗粒还表现出更高的变性抑制率，IC值为6.66 μg/mL，表明具有很强的抗炎作用。这些增强的能力开启了生物启发合成氧化镁纳米颗粒生物应用的新时代。因此，需要进一步研究来评估这些纳米颗粒的动力学性质。
