State Key Laboratory of Rice Biology, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Department of Plant Pathology, Faculty of Agriculture, Minia University, Minya 61519, Egypt.
Biomed Res Int. 2019 Feb 17;2019:5620989. doi: 10.1155/2019/5620989. eCollection 2019.
Recently, the use of herbs in the agriculture and food industry has increased significantly. In particular, L. extracts have been reported to have strong antibacterial properties, which depend on their chemical composition. The present study displayed a biological method for synthesis of magnesium oxide (MgO) nano-flowers. The nano-flowers are developed without using any catalyst agent. Aqueous Rosemary extract was used to synthesize MgO nano-flowers (MgONFs) in stirring conditions and temperature at 70°C for 4 h. The mixture solution was checked by UV-Vis spectrum to confirm the presence of nanoparticles. The MgO nano-flowers powder was further characterized in this study by the X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. In addition, bacteriological tests indicated that MgO nano-flowers significantly inhibited bacterial growth, biofilm formation, and motility of pv. , which is the causal agent of bacterial blight disease in rice. The electronic microscopic observation showed that bacterial cell death may be mainly due to destroy of cell integrity, resulting in leakage of intracellular content. As recommended, the use of Rosemary extract is an effective and green way to produce the MgO nano-flowers, which can be widely used in agricultural fields to suppress bacterial infection.
最近,草药在农业和食品工业中的使用显著增加。特别是,已报道迷迭香提取物具有很强的抗菌性能,这取决于其化学成分。本研究展示了一种生物合成方法,用于合成氧化镁(MgO)纳米花。纳米花的合成无需使用任何催化剂。在搅拌条件下,将迷迭香提取物水溶液在 70°C 下反应 4 小时,制备 MgO 纳米花(MgONFs)。通过紫外可见光谱检查混合物溶液以确认纳米颗粒的存在。在这项研究中,进一步通过 X 射线衍射、扫描电子显微镜、透射电子显微镜和傅里叶变换红外光谱对 MgO 纳米花粉末进行了表征。此外,细菌学测试表明,MgO 纳米花显著抑制了 pv. 的生长、生物膜形成和运动,pv. 是水稻细菌性枯萎病的病原体。电子显微镜观察表明,细菌细胞的死亡可能主要是由于细胞完整性的破坏,导致细胞内物质的泄漏。因此,建议使用迷迭香提取物是一种有效且绿色的方法来生产 MgO 纳米花,可广泛应用于农业领域,抑制细菌感染。