Shen Liming, Zhao Danqing, Chen Youjiao, Zhang Kaoyuan, Chen Xinqian, Lin Jing, Li Cuihua, Iqbal Javed, Zhao Yuxi, Liang Yi, Wei Yan, Feng Chengyun
College of Life Science and Oceanography, Shenzhen University, Shenzhen, 518060, P. R. China.
Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, P. R. China.
Proteomics Clin Appl. 2019 Sep;13(5):e1800060. doi: 10.1002/prca.201800060. Epub 2019 Jul 3.
This study is designed to screen serum proteins that may serve as biomarkers for gestational diabetes mellitus (GDM), and clarify the mechanisms of disease.
By using isobaric tags for relative and absolute quantitation proteomics analysis, serum proteins levels were quantified in pregnant women who subsequently developed GDM (12-16 weeks), GDM patients (24-28 weeks), and their corresponding controls. The strategy of mixing samples is used in proteomic analysis.
Thirty-one and 27 differentially expressed proteins are identified in the serum of pregnant women with developed GDM at 12-16 weeks and GDM patients during 24-28 weeks, respectively. Thirty eight and 28 proteins are identified in 24-28 weeks versus 12-16 weeks controls (24/12 CTR group), and 24-28 weeks GDM patients versus 12-16 weeks women with subsequently developed GDM (24/12 GDM group), respectively. Most of these proteins in the case and control subjects are associated with diabetes and maternal and perinatal short- and long-term complications.
The results highlight the roles of complement system and the blood clotting cascade in the pathogenesis of GDM. Differentially expressed proteins may serve as potential biomarkers for GDM prediction and diagnosis in the future.
本研究旨在筛选可能作为妊娠期糖尿病（GDM）生物标志物的血清蛋白，并阐明疾病机制。
采用相对和绝对定量蛋白质组学分析的等压标签，对随后发生GDM的孕妇（12 - 16周）、GDM患者（24 - 28周）及其相应对照组的血清蛋白水平进行定量。蛋白质组分析采用混合样本策略。
在12 - 16周发生GDM的孕妇血清和24 - 28周的GDM患者血清中分别鉴定出31种和27种差异表达蛋白。在24 - 28周对照组与12 - 16周对照组（24/12 CTR组）以及24 - 28周GDM患者与12 - 16周随后发生GDM的孕妇（24/12 GDM组）中分别鉴定出38种和28种蛋白。病例组和对照组中的这些蛋白大多与糖尿病以及母婴围产期短期和长期并发症相关。
结果突出了补体系统和凝血级联反应在GDM发病机制中的作用。差异表达蛋白未来可能作为GDM预测和诊断的潜在生物标志物。
