Ravindranath Adarsh Lalitha, Shariatdoust Mirali Seyed, Mathew Samuel, Gordon Reuven
Opt Express. 2019 May 27;27(11):16184-16194. doi: 10.1364/OE.27.016184.
Double-nanoholes fabricated by colloidal lithography were used for trapping single colloidal particles and single proteins. A gap separation of 60 nm between the cusps of the double-nanohole was achieved in a gold film of 70 nm thickness sputter coated onglass. The cusp separation was reduced steadily down to 10 nm by plasma etching the colloidal particles prior to sputter coating. Scanning electron microscopy was used to locate a particular double-nanohole and it was registered for later microscopy experiments. 30 nm polystyrene particles, the rubisco protein and bovine serum albumin were trapped using a laser focused through the aperture. Compared to other methods that require top-down nanofabrication, this approach is inexpensive and produces high-quality samples.
通过胶体光刻制造的双纳米孔用于捕获单个胶体颗粒和单个蛋白质。在溅射涂覆在玻璃上的70nm厚的金膜中，双纳米孔的尖端之间实现了60nm的间隙分离。通过在溅射涂覆之前对胶体颗粒进行等离子体蚀刻，尖端间距稳定地减小至10nm。使用扫描电子显微镜定位特定的双纳米孔，并将其记录下来用于后续的显微镜实验。使用通过孔径聚焦的激光捕获30nm聚苯乙烯颗粒、核酮糖-1,5-二磷酸羧化酶/加氧酶蛋白和牛血清白蛋白。与其他需要自上而下的纳米制造方法相比，这种方法成本低廉且能产生高质量的样品。
