Department of Electrical and Computer Engineering, University of Victoria, Victoria, BC, Canada V8W 3P6.
Lab Chip. 2013 Jul 7;13(13):2563-8. doi: 10.1039/c3lc00003f. Epub 2013 Feb 22.
A double nanohole in a metal film can optically trap nanoparticles such as polystyrene/silica spheres, encapsulated quantum dots and up-converting nanoparticles. Here we study the dynamics of trapped particles, showing a skewed distribution and low roll-off frequency that are indicative of Kramers-hopping at the nanoscale. Numerical simulations of trapped particles show a double-well potential normally found in Kramers-hopping systems, as well as providing quantitative agreement with the overall trapping potential. In addition, we demonstrate co-trapping of bovine serum albumin (BSA) with anti-BSA by sequential delivery in a microfluidic channel. This co-trapping opens up exciting possibilities for the study of protein interactions at the single particle level.
双纳米孔金属膜可以光学捕获纳米颗粒，如聚苯乙烯/二氧化硅球、封装量子点和上转换纳米颗粒。在这里，我们研究了被捕获粒子的动力学，表现出倾斜分布和低滚降频率，这表明在纳米尺度上存在 Kramers 跳跃。被捕获粒子的数值模拟显示出 Kramers 跳跃系统中通常存在的双势阱，并且与整体捕获势定量一致。此外，我们还通过在微流道中顺序输送，演示了牛血清白蛋白 (BSA) 与抗 BSA 的共捕获。这种共捕获为在单个颗粒水平上研究蛋白质相互作用开辟了令人兴奋的可能性。
