Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Napoli, Italy.
US Forest Service Rocky Mountain Research Station, Shrub Sciences Laboratory, Provo, USA.
Nat Prod Res. 2021 Jan;35(1):99-107. doi: 10.1080/14786419.2019.1614583. Epub 2019 Jun 5.
Radicinin is a phytotoxic fungal dihydropyranopyran-4,5-dione under evaluation for the development of a target-specific bioherbicide for invasive buffelgrass () control. It has already demonstrated high toxicity on host plants, low toxicity to native plants and no negative effects on zebrafish embryos. To continue these studies at the whole-plant level there is a need to obtain much larger quantities of radicinin, either by optimizing its large-scale production by fungal fermentation or through its total stereoselective synthesis. A rapid and sensitive HPLC method for quantification of radicinin in complex mixtures has been developed in order to evaluate its production by different strains and in different cultural conditions. The analysis proved that radicinin is not produced by all the strains tested and its synthesis is strongly affected by cultural conditions. The HPLC method could be useful in selecting the best fungal source for the production of this promising potential bioherbicide.
瑞迪辛因是一种真菌二氢吡喃并[4,5-d]酮，具有植物毒性，目前正在评估其作为一种针对入侵型象草（）的靶向生物除草剂的开发。它已经在宿主植物上表现出了很高的毒性，对本地植物的毒性较低，对斑马鱼胚胎也没有负面影响。为了在全植物水平上继续进行这些研究，需要通过真菌发酵来优化其大规模生产，或者通过其全立体选择性合成来获得更多的瑞迪辛因。为了评估不同菌株和不同培养条件下瑞迪辛因的产量，我们开发了一种快速灵敏的 HPLC 定量方法来检测复杂混合物中的瑞迪辛因。分析表明，并非所有测试的菌株都能产生瑞迪辛因，其合成受到培养条件的强烈影响。该 HPLC 方法可用于选择最适合生产这种有前途的潜在生物除草剂的真菌来源。
