Ulitzur S
Biochim Biophys Acta. 1979 Feb 26;572(2):211-7. doi: 10.1016/0005-2760(79)90036-5.
A new bioassay for lipase utilizes a dim mutant of luminous bacteria which emit light upon the addition of long chain fatty acids, especially myristic acid. The luminescence response is proportional to the amount of added myristic acid over a 100-fold range, down to 10 nM. Trimyristin was used as a substrate for lipase and the hydrolyzed myristic acid was determined by the response of the luminous bacteria either on a continuous basis in the same reaction mixture or alternatively, when the hydrolytic stage is done separately followed by the independent detecting system. Using these procedures it is possible to assay lipase activity at rate corresponding to a release of as low as 10 pmol myristic acid per min.
一种新的脂肪酶生物测定法利用了发光细菌的双突变体，这种突变体在添加长链脂肪酸（尤其是肉豆蔻酸）时会发光。在100倍的范围内，发光响应与添加的肉豆蔻酸量成正比，低至10 nM。三肉豆蔻精被用作脂肪酶的底物，水解产生的肉豆蔻酸通过发光细菌的响应来测定，既可以在同一反应混合物中连续进行，也可以在水解阶段单独完成后，通过独立的检测系统进行测定。使用这些方法，可以以每分钟低至10 pmol肉豆蔻酸释放量的速率测定脂肪酶活性。
