基于60 kDa蛋白酶基因限制性片段长度多态性分析的阴道毛滴虫临床分离株的遗传多样性
Genetic Diversity of Trichomonas Vaginalis Clinical Isolates According to Restriction Fragment Length Polymorphism Analysis of the 60-kDa Proteinase Gene.
作者信息
Hernández Hilda, Fraga Jorge, Marcet Ricardo, Alba Annia, Figueredo Mabel, Alfonso Yenisey, Rojas Lázara, Sarracent Jorge
机构信息
Parasitology Department, Institute of Tropical Medicine "Pedro Kouri", A.P. 601. Marianao 13, Havana, Cuba.
出版信息
Acta Parasitol. 2019 Jun;64(2):300-307. doi: 10.2478/s11686-019-00065-5. Epub 2019 Jun 5.
INTRODUCCTION
Trichomonas vaginalis is a highly prevalent parasitic that causes the sexually transmitted disease trichomoniasis with some serious health complications. More understanding about genetic features of the parasite can be helpful in the study of the pathogenesis, epidemiology of the infection and drug susceptibility. For this end, we conducted analysis of a fragment (23 kDa) of the p60 of T. vaginalis gene.
MATERIAL AND METHODS
The restriction fragment length polymorphism (RFLP) methods was used.
RESULT AND DISCUSSION
RFLP analysis showed the difference between T. vaginalis isolates from symptomatic and asymptomatic patients, suggesting a relation between the genetic identity of the isolates and their clinical manifestations.
引言
阴道毛滴虫是一种高度流行的寄生虫，可引起性传播疾病滴虫病，并伴有一些严重的健康并发症。对该寄生虫基因特征的更多了解有助于研究其发病机制、感染流行病学和药物敏感性。为此，我们对阴道毛滴虫基因p60的一个片段（23 kDa）进行了分析。
材料与方法
采用限制性片段长度多态性（RFLP）方法。
结果与讨论
RFLP分析显示有症状和无症状患者的阴道毛滴虫分离株之间存在差异，表明分离株的基因特征与其临床表现之间存在关联。
Zotero 插件