Yang Juan, Wang Guo-Quan, Zhou Qiong, Lu Wen, Ma Jun-Qing, Huang Jing-Hua
College of Agriculture, Guangxi University, Nanning, 530004, Guangxi, China.
Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, National Demonstration Center for Experimental Plant Science Education, Guangxi University, Nanning, 530004, Guangxi, China.
Exp Appl Acarol. 2019 Jun;78(2):273-293. doi: 10.1007/s10493-019-00387-z. Epub 2019 Jun 5.
Tetranychus urticae (Acari: Tetranychidae) is an extremely serious cassava (Manihot esculenta) pest. Building a genomic resource to investigate the molecular mechanisms of cassava responses to T. urticae is vital for characterizing cassava resistance to mites. Based on the tolerance of cassava varieties to mite infestation (focusing on mite development rate, fecundity and physiology), cassava variety SC8 was selected to analyze transcriptomic and proteomic changes after 5 days of T. urticae feeding. Transcriptomic analysis revealed 698 and 2140 genes with significant expression changes under low and high mite infestation, respectively. More defense-related genes were found in the enrichment pathways at high mite density than at low density. In addition, iTRAQ-labeled proteomic analysis revealed 191 proteins with significant expression changes under low mite infestation. Differentially expressed genes and proteins were mainly found in the following defense-related pathways: flavonoid biosynthesis, phenylpropanoid biosynthesis, and glutathione metabolism under low-density mite feeding and plant hormone signal transduction and plant-pathogen interaction pathways under high-density mite feeding. The plant hormone signal transduction network, involving ethylene, jasmonic acid, and salicylic acid transduction pathways, was explored in relation to the M. esculenta response to T. urticae. Correlation analysis of the transcriptome and proteome generated a Pearson correlation coefficients of R = 0.2953 (P < 0.01), which might have been due to post-transcriptional or post-translational regulation resulting in many genes being inconsistently expressed at both the transcript and protein levels. In summary, the M. esculenta transcriptome and proteome changed in response to T. urticae, providing insight into the general activation of plant defense pathways in response to mite infestation.
二斑叶螨（蜱螨亚纲：叶螨科）是木薯（Manihot esculenta）极其严重的害虫。构建基因组资源以研究木薯对二斑叶螨反应的分子机制对于表征木薯对螨类的抗性至关重要。基于木薯品种对螨类侵染的耐受性（重点关注螨类发育速率、繁殖力和生理学），选择木薯品种SC8分析二斑叶螨取食5天后的转录组和蛋白质组变化。转录组分析显示，在低螨侵染和高螨侵染下分别有698个和2140个基因表达发生显著变化。在高螨密度下的富集途径中发现的与防御相关的基因比低螨密度下更多。此外，iTRAQ标记的蛋白质组分析显示，在低螨侵染下有191种蛋白质表达发生显著变化。差异表达的基因和蛋白质主要存在于以下与防御相关的途径中：低密度螨取食时的类黄酮生物合成、苯丙烷生物合成和谷胱甘肽代谢，以及高密度螨取食时的植物激素信号转导和植物 - 病原体相互作用途径。研究了涉及乙烯、茉莉酸和水杨酸转导途径的植物激素信号转导网络与木薯对二斑叶螨的反应之间的关系。转录组和蛋白质组的相关性分析产生的皮尔逊相关系数R = 0.2953（P < 0.01），这可能是由于转录后或翻译后调控导致许多基因在转录和蛋白质水平上的表达不一致。总之，木薯的转录组和蛋白质组因二斑叶螨而发生变化，为深入了解植物防御途径在应对螨类侵染时的一般激活情况提供了线索。
