Liang Xiao, Chen Qing, Lu Hui, Wu Chunling, Lu Fuping, Tang Jihong
Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, Hainan, China.
Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, Hainan, China.
Exp Appl Acarol. 2017 Mar;71(3):195-209. doi: 10.1007/s10493-017-0125-y. Epub 2017 Apr 12.
In order to study the function of peroxidase (POD) and polyphenol oxidase (PPO) in cassava resistance to spider mites, we tested the changes of transcription levels and activities of these two protective enzymes in both cassava and Tetranychus urticae (=T. cinnabarinus) during the interaction. The results showed that after damage of the mite-susceptible cassava cultivar BRA900 by T. urticae for 1 and 8 days, the transcription levels of MePOD and MePPO and the activities of POD and PPO showed no significant difference compared with those in undamaged leaves. However, the corresponding transcription levels and activities in 1- and 8-day-damaged leaves of mite-resistant cassava cultivar C1115 increased to a significant level of approximately twofold. When T. urticae fed on BRA900 for 1 and 8 days, the transcription levels of TcPPO and TcPOD and the activities of PPO and POD showed no significant difference compared with those before feeding. However, the corresponding transcription levels and activities of these two protective enzymes in T. urticae feeding on C1115 significantly decreased by about half. This study preliminarily validates the function of POD and PPO in cassava resistance to T. urticae, and provides candidate gene resource for molecular breeding of spider mite-resistant cassava.
为研究过氧化物酶（POD）和多酚氧化酶（PPO）在木薯对朱砂叶螨抗性中的作用，我们检测了这两种保护酶在木薯与朱砂叶螨（= 朱砂叶螨）互作过程中的转录水平和活性变化。结果表明，感螨木薯品种BRA900在被朱砂叶螨侵害1天和8天后，MePOD和MePPO的转录水平以及POD和PPO的活性与未受损叶片相比无显著差异。然而，抗螨木薯品种C1115在被侵害1天和8天的叶片中，相应的转录水平和活性显著增加至约两倍。当朱砂叶螨取食BRA900 1天和8天时，TcPPO和TcPOD的转录水平以及PPO和POD的活性与取食前相比无显著差异。然而，取食C1115的朱砂叶螨中这两种保护酶的相应转录水平和活性显著降低了约一半。本研究初步验证了POD和PPO在木薯对朱砂叶螨抗性中的作用，并为抗螨木薯分子育种提供了候选基因资源。
