Department Clinical Pharmacy and Pharmacology, University of Groningen, Groningen, the Netherlands.
Department of Phthisiology and Pulmonology, Grodno State Medical University, Grodno, Belarus.
Ther Drug Monit. 2019 Dec;41(6):714-718. doi: 10.1097/FTD.0000000000000659.
Dried blood spot (DBS) sampling is a blood collection tool that uses a finger prick to obtain a blood drop on a DBS card. It can be used for therapeutic drug monitoring, a method that uses blood drug concentrations to optimize individual treatment. DBS sampling is believed to be a simpler way of blood collection compared with venous sampling. The aim of this study was to evaluate the quality of DBSs from patients with tuberculosis all around the world based on quality indicators in a structured assessment procedure.
Total 464 DBS cards were obtained from 4 countries: Bangladesh, Belarus, Indonesia, and Paraguay. The quality of the DBS cards was assessed using a checklist consisting of 19 questions divided into 4 categories: the integrity of the DBS materials, appropriate drying time, blood volume, and blood spot collection.
After examination, 859 of 1856 (46%) blood spots did not comply with present quality criteria. In 625 cases (34%), this was due to incorrect blood spot collection. The DBS cards from Bangladesh, Indonesia, and Paraguay seemed to be affected by air humidity, causing the blood spots not to dry appropriately.
New tools to help obtain blood spots of sufficient quality are necessary and environmental specific recommendations to determine plasma concentration correctly. In addition, 3% of the DBS cards were rejected because the integrity of the materials suggesting that the quality of plastic ziplock bags currently used to protect the DBS cards against contamination and humidity may not be sufficient.
干血斑（DBS）采样是一种使用指尖采血获取 DBS 卡上血斑的血液采集工具。它可用于治疗药物监测，这是一种使用血液药物浓度来优化个体治疗的方法。与静脉采样相比，DBS 采样被认为是一种更简单的采血方式。本研究的目的是基于结构化评估程序中的质量指标，评估来自世界各地结核病患者的 DBS 的质量。
共从孟加拉国、白俄罗斯、印度尼西亚和巴拉圭 4 个国家获得 464 张 DBS 卡。使用由 19 个问题组成的检查表评估 DBS 卡的质量，该检查表分为 4 类：DBS 材料的完整性、适当的干燥时间、血量和血斑采集。
检查后，1856 个血斑中的 859 个（46%）不符合当前的质量标准。在 625 例（34%）中，这是由于血斑采集不正确。来自孟加拉国、印度尼西亚和巴拉圭的 DBS 卡似乎受到空气湿度的影响，导致血斑不能适当干燥。
需要新的工具来帮助获得足够质量的血斑，并针对特定环境确定正确的血浆浓度。此外，由于材料完整性的原因，3%的 DBS 卡被拒收，这表明目前用于防止 DBS 卡受到污染和湿度影响的塑料拉链袋的质量可能不足。
