Research and Development Department, Farin Behbood Tashkhis LTD, Tehran, Iran.
Department of Medicinal Chemistry, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran; Biosensor Research Center (BRC), Ardabil University of Medical Sciences, Ardabil, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Nov 5;222:117218. doi: 10.1016/j.saa.2019.117218. Epub 2019 May 29.
In this work, we demonstrated the development of a colorimetric immunosensor using surface plasmon resonance band of gold nanoparticles for the detection of prostate specific antigen (PSA). To develop this biosensing tool, triangular gold nanoparticles (AuNPs) were synthesized using Tween-20 as a nonionic surfactant and then, conjugated with PSA capture antibody (Ab-AuNPs). When exposed to Ab-AuNPs, PSA antigens were found to be successfully captured by nanosystem (PSA)-Ab-AuNPs. Next, (PSA)-Ab-AuNPs were incubated with second PSA antibody (2)-decorated magnetite (FeO-Ab) and separated by an external magnetic force to leave Ab-AuNPs in the supernatant solution to be directly analyzed using UV-Vis spectroscopy. It was found that the absorption intensity was directly proportional to the PSA concentration. As a result, the linear range for PSA detection was found to be 0.01-20 ng mL with a detection limit of 0.009 ng mL. Because of significant stability of the prepared Ab-AuNPs and excellent selectivity to the PSA antigen, this simple and sensitive sensing system is proposed to be potentially effective in the fast and real-time analysis of clinical samples from prostate cancer patients. We believe that the simple platform of this immunosensor to be useful in the development of future point-of-care sensing tools, working on the quantification of biomarkers in a drop of blood.
在这项工作中，我们展示了一种使用金纳米粒子的表面等离子体共振带的比色免疫传感器的开发，用于检测前列腺特异性抗原（PSA）。为了开发这种生物传感工具，使用吐温-20 作为非离子表面活性剂合成了三角形金纳米粒子（AuNPs），然后与 PSA 捕获抗体（Ab-AuNPs）偶联。当暴露于 Ab-AuNPs 时，发现 PSA 抗原被纳米系统（PSA）-Ab-AuNPs 成功捕获。接下来，（PSA）-Ab-AuNPs 与第二 PSA 抗体（2）修饰的磁铁矿（FeO-Ab）孵育，并通过外部磁力分离，将 Ab-AuNPs 留在上清液中，直接使用紫外-可见光谱进行分析。结果发现，吸收强度与 PSA 浓度成正比。因此，发现 PSA 检测的线性范围为 0.01-20ng/mL，检测限为 0.009ng/mL。由于制备的 Ab-AuNPs 具有显著的稳定性和对 PSA 抗原的优异选择性，因此提出了这种简单灵敏的传感系统，有望在快速实时分析前列腺癌患者的临床样本中发挥作用。我们相信，这种免疫传感器的简单平台将有助于未来的即时护理传感工具的开发，用于定量血液中的生物标志物。
