基于纳米氧化锌金属有机框架的光学生物传感器用于前列腺特异性抗原监测。
Prostate-Specific Antigen Monitoring Using Nano Zinc(II) Metal-Organic Framework-Based Optical Biosensor.
机构信息
Department of Nanomaterials and Nanotechnology, Central Metallurgical R & D Institute, Cairo 11421, Egypt.
Department of Inorganic Chemistry, National Research Centre, Cairo 12622, Egypt.
出版信息
Biosensors (Basel). 2022 Oct 27;12(11):931. doi: 10.3390/bios12110931.
BACKGROUND
The prostate-specific antigen (PSA) is an important cancer biomarker that is commonly utilized in the diagnosis of prostate cancer. The development of a PSA determination technique that is rapid, simple, and inexpensive, in addition to highly accurate, sensitive, and selective, remains a formidable obstacle.
METHODS
In this study, we developed a practical biosensor based on Zn(II) metal-organic framework nanoparticles (Zn-MOFs-NPs). Many spectroscopic and microanalytical tools are used to determine the structure, morphology, and physicochemical properties of the prepared MOF.
RESULTS
According to the results, Zn-MOFs-NPs are sensitive to PSA, selective to an extremely greater extent, and stable in terms of chemical composition. Furthermore, the Zn-MOFs-NPs did not exhibit any interferences from other common analytes that might cause interference. The detection limit for PSA was calculated and was 0.145 fg/mL throughout a wide linear concentration range (0.1 fg/mL-20 pg/mL).
CONCLUSIONS
Zn-MOFs-NPs were successfully used as a growing biosensor for the monitoring and measurement of PSA in biological real samples.
背景
前列腺特异性抗原（PSA）是一种重要的癌症生物标志物，常用于前列腺癌的诊断。除了高度准确、灵敏和选择性之外，开发一种快速、简单、廉价的 PSA 测定技术仍然是一个艰巨的挑战。
方法
在本研究中，我们开发了一种基于 Zn(II) 金属有机骨架纳米粒子（Zn-MOFs-NPs）的实用生物传感器。使用多种光谱和微分析工具来确定所制备的 MOF 的结构、形态和物理化学性质。
结果
结果表明，Zn-MOFs-NPs 对 PSA 具有敏感性，对 PSA 具有极高的选择性和稳定性，并且在化学成分方面表现稳定。此外，Zn-MOFs-NPs 没有表现出任何可能引起干扰的其他常见分析物的干扰。通过计算得出 PSA 的检测限为 0.145 fg/mL，在很宽的线性浓度范围内（0.1 fg/mL-20 pg/mL）。
结论
Zn-MOFs-NPs 成功地用作监测和测量生物实际样本中 PSA 的生长型生物传感器。
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