Komba Shiro, Kotake-Nara Eiichi, Tsuzuki Wakako
Food Component Analysis Unit, Food Research Institute, National Agriculture and Food Research Organization, 2-1-12, Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Metabolites. 2019 Jun 6;9(6):107. doi: 10.3390/metabo9060107.
We succeeded in simultaneously synthesizing the vitamin D family, vitamins D, D, D, D, and D, from β-sitosterol, which is sold as a commercially available reagent from Tokyo Chemical Industry Co., Ltd. It is officially sold as a mixture of four phytosterols {β-sitosterol (40-45%), campesterol (20-30%), stigmasterol, and brassicasterol}. Owing to this, we anticipated that, using this reagent, various vitamin D analogs could be synthesized simultaneously. We also synthesized vitamin D from pure cholesterol and analyzed and compared all vitamin D analogs (D, D, D, D, D, and D) by HSQC NMR. We succeeded in clearly demonstrating the difference in the NMR chemical shifts for each vitamin D analog.
我们成功地从东京化学工业株式会社作为市售试剂出售的β-谷甾醇中同时合成了维生素D家族，即维生素D、D、D、D和D。它作为四种植物甾醇的混合物正式出售{β-谷甾醇（40 - 45%）、菜油甾醇（20 - 30%）、豆甾醇和油菜甾醇}。因此，我们预计，使用这种试剂，可以同时合成各种维生素D类似物。我们还从纯胆固醇中合成了维生素D，并通过HSQC NMR对所有维生素D类似物（D、D、D、D、D和D）进行了分析和比较。我们成功地清楚证明了每种维生素D类似物在NMR化学位移上的差异。
