Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del-Rei, Divinópolis, Minas Gerais, Brazil.
Departamento de Química, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Nat Prod Res. 2021 May;35(10):1676-1681. doi: 10.1080/14786419.2019.1624955. Epub 2019 Jun 7.
Dichloromethane (DCM) fraction and sub-fractions obtained from leaves were examined in order to determine their phytotoxic and antioxidant effects. The dichloromethane fraction was submitted to a preparative layer chromatography leading to seven sub-fractions (DCM1-DCM7). Gas chromatography-mass spectrometry (GC-MS) was performed on the dichloromethane sub-fractions. The DCM sub-fractions presented phytotoxic potential; at a concentration of 125 µg per plate, DCM6 and DCM4 showed the strongest results on and respectively. The DCM fraction and DCM4 sub-fraction were more effective than 2,6-di-tert-butyl-4-methylphenol (BHT) at scavenging the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Analysis by GC-MS showed the presence of methyl palmitate (33.05%) in DCM4 and methyl palmitate (17.29%) and methyl oleate (50.96%) in DCM6, suggesting that the activities exhibited by the sub-fractions may be attributed, at least partially, to these major compounds. These results indicate that the DCM sub-fractions of could be used as natural herbicides and antioxidants.
为了确定其植物毒性和抗氧化作用，对 叶片得到的二氯甲烷（DCM）馏分和亚馏分进行了研究。将二氯甲烷馏分进行制备性层析，得到七个亚馏分（DCM1-DCM7）。对二氯甲烷亚馏分进行气相色谱-质谱（GC-MS）分析。二氯甲烷亚馏分表现出植物毒性潜力；在 125μg/板的浓度下，DCM6 和 DCM4 分别对 和 表现出最强的效果。DCM 馏分和 DCM4 亚馏分在清除 1,1-二苯基-2-苦基肼（DPPH）自由基方面比 2,6-二叔丁基-4-甲基苯酚（BHT）更有效。GC-MS 分析表明 DCM4 中存在棕榈酸甲酯（33.05%），DCM6 中存在棕榈酸甲酯（17.29%）和油酸甲酯（50.96%），这表明亚馏分表现出的活性至少部分归因于这些主要化合物。这些结果表明， 叶片的 DCM 亚馏分可用作天然除草剂和抗氧化剂。
