从贝宁屠宰动物中分离出的粪便沙门氏菌属的耐药性和毒力决定因素。
Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin.
作者信息
Deguenon Esther, Dougnon Victorien, Lozes Evelyne, Maman Nana, Agbankpe Jerrold, Abdel-Massih Roula M, Djegui Fidélia, Baba-Moussa Lamine, Dougnon Jacques
机构信息
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 PO Box 2009, Cotonou, Benin.
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, UAC, 05 PO Box 1604, Cotonou, Benin.
出版信息
BMC Res Notes. 2019 Jun 7;12(1):317. doi: 10.1186/s13104-019-4341-x.
OBJECTIVE
Salmonella spp. are one of the leading foodborne pathogens worldwide naturally found in the intestines of many animals. People that are in direct contact with the infected animals or their cages may become ill. The aim of this study was to determine the prevalence, antibiogram and virulence genes associated with Salmonella serovars from fecal samples of animals intended for consumption in Southern Benin.
RESULTS
Out of a total of 406 samples, 2.46% were positive. The isolates identified were multidrug-resistant Salmonella spp. to penicillins, first generation cephalosporins and some aminoglycosides. All Salmonella isolates produced invA gene of 284 bp, fimA of 85 bp and stn of 260 bp. The spvC gene (571 bp) was present in 10% of the isolates whereas the spvR gene (310 bp) was found in 20% of the isolates. The control strain possessed all the tested genes. The invA gene implies that strains are able to invade epithelial cells. The fimA and stn genes present in all isolates show that they are capable of causing gastrointestinal illness in humans. The presence of spvC and spvR genes suggests the possibility of these strains to produce toxins.
目的
沙门氏菌属是全球主要的食源性病原体之一，天然存在于许多动物的肠道中。直接接触受感染动物或其笼子的人可能会生病。本研究的目的是确定来自贝宁南部供食用动物粪便样本中与沙门氏菌血清型相关的流行率、抗菌谱和毒力基因。
结果
在总共406份样本中，2.46%呈阳性。鉴定出的分离株为对青霉素、第一代头孢菌素和一些氨基糖苷类药物具有多重耐药性的沙门氏菌属。所有沙门氏菌分离株均产生284 bp的invA基因、85 bp的fimA基因和260 bp的stn基因。10%的分离株中存在spvC基因（571 bp），而20%的分离株中发现了spvR基因（310 bp）。对照菌株拥有所有测试基因。invA基因表明菌株能够侵入上皮细胞。所有分离株中存在的fimA和stn基因表明它们能够在人类中引起胃肠道疾病。spvC和spvR基因的存在表明这些菌株有可能产生毒素。
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