克氏锥蝽中花生四烯酸的存在情况。

The occurrence of arachidonic acid in Triatoma infestans.

Brenner R R, Bernasconi A M

Instituto de Investigaciones Bioquímicas de La Plata, Facultad de Ciencias Médicas, Argentina.

Comp Biochem Physiol B. 1987;87(4):815-9. doi: 10.1016/0305-0491(87)90394-4.

Abstract

Gas-liquid chromatography and mass spectrometry were used to verify the presence of arachidonic acid (20:4 omega 6) in adult male and female organs of the blood-suckling bug Triatoma infestans. Fat body, gonad and head lipids were analyzed. 2. Male gonads contained the highest percentage of phospholipids and the highest percentage of arachidonic acid. 3. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and sphingomyelin were isolated from gonads and heads of sexed insects and the fatty acid composition analyzed. 4. All the phospholipids analyzed contained relevant percentages of linoleic acid and fair percentages of arachidonic acid, except for sphingomyelin where no 20:4 omega 6 acid was found. 5. However, arachidonic acid was specifically incorporated in phosphatidylinositol since this phospholipid contained very large percentages of the acid. 6. In male gonads, phosphatidylcholine also contained a similar high percentage of the acid. Arachidonic acid is apparently incorporated, selectively, into these lipids from the blood of the host.

摘要

采用气液色谱法和质谱分析法，以验证吸血蝽（大锥蝽）成年雄性和雌性器官中花生四烯酸（20:4 ω6）的存在情况。对脂肪体、性腺和头部脂质进行了分析。2. 雄性性腺中磷脂百分比最高，花生四烯酸百分比也最高。3. 从已区分性别的昆虫的性腺和头部中分离出磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰丝氨酸和鞘磷脂，并分析其脂肪酸组成。4. 除鞘磷脂中未发现20:4 ω6酸外，所有分析的磷脂均含有相应百分比的亚油酸和一定百分比的花生四烯酸。5. 然而，花生四烯酸特异性地掺入磷脂酰肌醇中，因为这种磷脂含有非常高比例的该酸。6. 在雄性性腺中，磷脂酰胆碱也含有相似的高比例该酸。花生四烯酸显然是从宿主血液中选择性地掺入这些脂质中的。