Li Shi-Yan, Huang Pin-Tong, Fang Yong, Wu Yao, Zhou Ling, Luo Jie-Li, Wang Xian-Chen, Chen Yun-Chong
Department of Ultrasound, Sir Run Run Shaw Hospital, Zhejiang University, School of Medicine, Hangzhou, Zhejiang Province, China.
Department of Ultrasound, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, Zhejiang Province, China.
Transl Oncol. 2019 Aug;12(8):1113-1121. doi: 10.1016/j.tranon.2019.05.007. Epub 2019 Jun 5.
Residual cancer after incomplete ablation remains a major problem for radiofrequency ablation (RFA). We aimed to investigate the synergetic treatment efficacy of RFA combined with ultrasonic cavitation for liver tumor. Sixty rabbits with VX2 liver tumor were randomly divided into three groups. Group A was control group without any treatment. Combined ultrasonic cavitation with RFA was performed for group B1. Group B2 underwent RFA alone. The histopathological results were compared at the 5th, 11th, and 18th day of experiment, and the survival time and metastasis were assessed. The tumor volume growth rate, percentage of necrosis area, microvessel density, and apoptosis index showed significant differences among these groups at the 5th day, 11th day, and 18th day of experiment (P < .05). In contrast, the difference of metastatic score was not significant at the 5th and 11th day (P > .05). At the 18th day, the metastatic score of group A was significant higher than that of group B1 (P < .05), whereas the differences between group A and group B2, or group B1 and group B2 were not significant (P > .05). The median/range interquartile of survival time in groups A, B1, and B2 were 25/8 days, 50/19 days, and 48/20 days, respectively, and there was significant difference between groups A and B1 or B2 (P < .05). The difference between groups B1 and B2 was not significant (P > .05). Ultrasonic cavitation after incomplete RFA for liver tumor improved the antitumor effect, which could be considered as a potentially useful combined therapeutic strategy for liver malignancy.
不完全消融后残留癌仍是射频消融（RFA）的一个主要问题。我们旨在研究RFA联合超声空化对肝肿瘤的协同治疗效果。将60只患有VX2肝肿瘤的兔子随机分为三组。A组为未接受任何治疗的对照组。B1组进行超声空化联合RFA。B2组单独进行RFA。在实验的第5天、第11天和第18天比较组织病理学结果，并评估生存时间和转移情况。在实验的第5天、第11天和第18天，这些组之间的肿瘤体积生长率、坏死面积百分比、微血管密度和凋亡指数显示出显著差异（P < 0.05）。相比之下，在第5天和第11天转移评分的差异不显著（P > 0.05）。在第18天，A组的转移评分显著高于B1组（P < 0.05），而A组与B2组之间或B1组与B2组之间的差异不显著（P > 0.05）。A组、B1组和B2组的生存时间中位数/四分位间距分别为25/8天、50/19天和48/20天，A组与B1组或B2组之间存在显著差异（P < 0.05）。B1组和B2组之间的差异不显著（P > 0.05）。肝肿瘤RFA不完全后进行超声空化可提高抗肿瘤效果，这可被视为一种对肝脏恶性肿瘤潜在有用的联合治疗策略。
