抗-D（Rh）抗体的人单克隆IgG1和IgG3亚类在介导红细胞与巨噬细胞结合能力方面的活性差异。
Differences between the activities of human monoclonal IgG1 and IgG3 subclasses of anti-D(Rh) antibody in their ability to mediate red cell-binding to macrophages.
作者信息
Wiener E, Atwal A, Thompson K M, Melamed M D, Gorick B, Hughes-Jones N C
机构信息
Department of Haematology, St. Mary's Hospital Medical School, London.
出版信息
Immunology. 1987 Nov;62(3):401-4.
Abstract
Four IgG1 and three IgG3 human monoclonal antibodies specific for the blood group D(Rh) antigen were tested for their ability to mediate red cell-binding to macrophages in vitro. The IgG3 monoclonals were found to opsonize at a density of approximately 100 molecules per red cell, whereas the IgG1 antibodies were only active at a level of 10,000 molecules per cell. There was no substantial difference between the two IgG subclasses in their ability to bind to Fc receptors on macrophages and it is suggested that the more potent opsonic activity of IgG3 is the result of the relatively long hinge, leading to greater accessibility to the Fc receptor binding site on the Fc piece.
摘要
对四种针对D（Rh）血型抗原的人源IgG1单克隆抗体和三种人源IgG3单克隆抗体进行了体外介导红细胞与巨噬细胞结合能力的测试。发现IgG3单克隆抗体以每红细胞约100个分子的密度进行调理作用，而IgG1抗体仅在每细胞10,000个分子的水平上具有活性。这两种IgG亚类在与巨噬细胞上Fc受体结合的能力上没有实质性差异，并且有人提出IgG3更强的调理活性是相对较长的铰链区的结果，这导致Fc片段上的Fc受体结合位点更容易接近。
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