抑制亮蛋白聚糖可抑制膀胱癌的增殖和迁移。
Knockdown of Lumican Inhibits Proliferation and Migration of Bladder Cancer.
作者信息
Mao Weipu, Luo Ming, Huang Xin, Wang Qian, Fan Jie, Gao Li, Zhang Yanxiang, Geng Jiang
机构信息
The People's Hospital of Yingshang, Anhui, 236200, China; Shanghai Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
Shanghai Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
出版信息
Transl Oncol. 2019 Aug;12(8):1072-1078. doi: 10.1016/j.tranon.2019.05.014. Epub 2019 Jun 6.
Lumican (LUM) is differentially expressed between normal and cancer tissues. The purpose of this study was to investigate the role of LUM in the proliferation and migration of bladder cancer (BCa) cells. Our study included 97 cases of BCa diagnosis from our hospital between June 2013 and June 2016. The expression of LUM was analyzed by immunohistochemistry and Western blot. To characterize the function of LUM, BCa cells were stably infected with a lentivirus against LUM, and cell proliferation, migration and cell cycle were investigated. In addition, xenograft experiments were performed in nude mice to evaluate the role of LUM in BCa. Our results showed that LUM was overexpressed in BCa tissues and cell lines in comparison to normal tissues. LUM expression was related to pathological type, T stage and N stage (P < .05). In addition, depletion of LUM inhibited the proliferation and migration of BCa cells by inactivating MAPK signaling. In conclusion, LUM promotes the proliferation and migration of BCa cells and may serve as a potential therapeutic target for BCa.
角膜光蛋白（LUM）在正常组织和癌组织中存在差异表达。本研究旨在探讨LUM在膀胱癌细胞（BCa）增殖和迁移中的作用。我们的研究纳入了2013年6月至2016年6月期间我院诊断的97例BCa病例。通过免疫组织化学和蛋白质印迹法分析LUM的表达。为了表征LUM的功能，用针对LUM的慢病毒稳定感染BCa细胞，并研究细胞增殖、迁移和细胞周期。此外，在裸鼠中进行异种移植实验以评估LUM在BCa中的作用。我们的结果表明，与正常组织相比，LUM在BCa组织和细胞系中过表达。LUM表达与病理类型、T分期和N分期相关（P<0.05）。此外，LUM的缺失通过使丝裂原活化蛋白激酶（MAPK）信号失活来抑制BCa细胞的增殖和迁移。总之，LUM促进BCa细胞的增殖和迁移，可能成为BCa的潜在治疗靶点。
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