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使用直线加速器进行体外放射生物学实验。

Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments.

作者信息

Hao Jing, Magnelli Anthony, Godley Andrew, Yu Jennifer S

机构信息

Department of Cancer Biology, Cleveland Clinic.

Department of Radiation Oncology, Cleveland Clinic.

出版信息

J Vis Exp. 2019 May 26(147). doi: 10.3791/59514.

DOI:10.3791/59514
PMID:31180366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7071788/
Abstract

Radiation therapy remains one of the cornerstones of cancer management. For most cancers, it is the most effective, nonsurgical therapy to debulk tumors. Here, we describe a method to irradiate cancer cells with a linear accelerator. The advancement of linear accelerator technology has improved the precision and efficiency of radiation therapy. The biological effects of a wide range of radiation doses and dose rates continue to be an intense area of investigation. Use of linear accelerators can facilitate these studies using clinically relevant doses and dose rates.

摘要

放射治疗仍然是癌症治疗的基石之一。对于大多数癌症来说，它是最有效的非手术肿瘤减容疗法。在此，我们描述一种使用直线加速器照射癌细胞的方法。直线加速器技术的进步提高了放射治疗的精度和效率。广泛的辐射剂量和剂量率的生物学效应仍然是一个深入研究的领域。使用直线加速器可以利用临床相关的剂量和剂量率来促进这些研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8e5/7071788/038a653e4d6b/nihms-1557754-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8e5/7071788/76d255b9b7e3/nihms-1557754-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8e5/7071788/038a653e4d6b/nihms-1557754-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8e5/7071788/76d255b9b7e3/nihms-1557754-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8e5/7071788/038a653e4d6b/nihms-1557754-f0002.jpg

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1
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J Vis Exp. 2019 May 26(147). doi: 10.3791/59514.
2
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Hypoxia imaging in upper gastrointestinal tumors and application to radiation therapy.上消化道肿瘤的缺氧成像及其在放射治疗中的应用
J Gastrointest Oncol. 2018 Dec;9(6):1044-1053. doi: 10.21037/jgo.2018.09.15.
2
Combination immunotherapy and radiation therapy strategies for pancreatic cancer-targeting multiple steps in the cancer immunity cycle.针对胰腺癌的联合免疫疗法和放射疗法策略——靶向癌症免疫循环中的多个步骤。
J Gastrointest Oncol. 2018 Dec;9(6):1014-1026. doi: 10.21037/jgo.2018.05.16.
3
MRI-Linear Accelerator Radiotherapy Systems.
微小RNA-148a增强直肠癌患者对放化疗的治疗反应并通过直接靶向促进细胞凋亡。
Biomedicines. 2021 Oct 1;9(10):1371. doi: 10.3390/biomedicines9101371.
磁共振引导直线加速器放射治疗系统。
Clin Oncol (R Coll Radiol). 2018 Nov;30(11):686-691. doi: 10.1016/j.clon.2018.08.003. Epub 2018 Sep 6.
4
The effects of extra high dose rate irradiation on glioma stem-like cells.超高剂量率照射对神经胶质瘤干细胞的影响。
PLoS One. 2018 Aug 17;13(8):e0202533. doi: 10.1371/journal.pone.0202533. eCollection 2018.
5
Amotosalen/UVA treatment inactivates T cells more effectively than the recommended gamma dose for prevention of transfusion-associated graft-versus-host disease.氨甲蝶呤/紫外线A疗法比预防输血相关移植物抗宿主病的推荐γ剂量更有效地使T细胞失活。
Transfusion. 2018 Jun;58(6):1506-1515. doi: 10.1111/trf.14589. Epub 2018 Apr 2.
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Radiation-induced G2/M arrest rarely occurred in glioblastoma stem-like cells.辐射诱导的G2/M期阻滞在胶质母细胞瘤干细胞样细胞中很少发生。
Int J Radiat Biol. 2018 Apr;94(4):394-402. doi: 10.1080/09553002.2018.1440094. Epub 2018 Feb 27.
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