Distler J, Ebert A, Mansouri K, Pissowotzki K, Stockmann M, Piepersberg W
Institut für Organische Chemie und Biochemie der Technischen Hochschule Darmstadt, FRG.
Nucleic Acids Res. 1987 Oct 12;15(19):8041-56. doi: 10.1093/nar/15.19.8041.
Three streptomycin (SM) production genes from Streptomyces griseus clustered around aphD, the major resistance gene, have been sequenced: strB, coding for an aminocyclitol amidinotransferase, ORF5 (strR), a putative regulatory gene, and ORF1 (strD), possibly coding for a hexose nucleotidylating enzyme. Three promoters and at least five, partially overlapping, transcripts have been identified by S1 mapping and Northern blot experiments. aphD, the resistance gene, is transcribed from two promoters. One of them, located inside the strR gene, seems to be constitutive and the other is switched on later in the growth phase. The late transcripts cover the resistance gene (aphD) and a regulatory gene (strR) which controls the expression of strB.
来自灰色链霉菌的三个围绕主要抗性基因aphD成簇的链霉素（SM）生产基因已被测序：strB，编码氨基环醇脒基转移酶；ORF5（strR），一个假定的调控基因；以及ORF1（strD），可能编码一种己糖核苷酸化酶。通过S1作图和Northern印迹实验已鉴定出三个启动子和至少五个部分重叠的转录本。抗性基因aphD从两个启动子转录。其中一个位于strR基因内部，似乎是组成型的，另一个在生长后期开启。后期转录本覆盖抗性基因（aphD）和一个控制strB表达的调控基因（strR）。
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