College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals , Shandong Normal University , Jinan , Shandong 250014 , China.
Anal Chem. 2019 Jun 18;91(12):7505-7509. doi: 10.1021/acs.analchem.9b01617. Epub 2019 Jun 5.
Accurate and sensitive detection of rare cancer cells such as circulating tumor cells (CTCs) has attracted great interest in the fields of clinical diagnosis and cancer research. However, the reported methods for cancer cell detection often involve a complicated platform and laborious procedures with a limited sensitivity. Herein, we construct a new entropy-driven DNA nanomachine for single-molecule detection of rare cancer cells. This assay employs the entropy-driven DNA nanomachine for efficient cancer cell recognition and signal amplification without the involvement of any expensive and unstable antibodies and enzymes, and it enables one-step detection of living cancer cells with high sensitivity and good specificity. This DNA nanomachine can be further applied for rare CTC detection in whole blood samples.
准确而灵敏地检测稀有癌细胞，如循环肿瘤细胞（CTCs），在临床诊断和癌症研究领域引起了极大的兴趣。然而，已报道的癌细胞检测方法通常涉及复杂的平台和繁琐的程序，并且灵敏度有限。在此，我们构建了一种新的基于熵驱动的 DNA 纳米机器，用于稀有癌细胞的单分子检测。该方法利用熵驱动的 DNA 纳米机器进行高效的癌细胞识别和信号放大，而不涉及任何昂贵且不稳定的抗体和酶，并且能够一步检测活癌细胞，具有高灵敏度和良好的特异性。这种 DNA 纳米机器还可以进一步应用于全血样本中稀有 CTC 的检测。
